| Pseudacidovorax intermedius NH-1, a novel marine nitrogen-fixing bacterium isolated from the South China Sea. | |
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MedLine Citation:
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PMID: 22806723 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have taxonomically and phylogenetically characterized a novel nitrogen-fixing bacterial strain, NH-1, which was recently isolated from surface sediments of the South China Sea. The presence of the nifH gene was determined by PCR amplification. The strain NH-1 was found to belong to the genus Pseudacidovorax based on phenotypic characterization, 16S rDNA sequencing, G+C content and DNA-DNA hybridization. Isolate NH-1 was identified as Pseudacidovorax intermedius. In addition, we investigated the links between environmental factors and the nitrogenase activity of NH-1. We found that the nitrogen fixation capacity of NH-1 varied strongly when cells were grown with different ammonium ion and oxygen concentrations, amino acids and carbohydrates. This is the first report of the isolation of Pseudacidovorax from the ocean and the first study to explore the effects of different culture conditions on the nitrogenase activities of the isolate. This study provides evidence that marine nitrogen-fixing microorganisms are far more diverse than currently recognized. |
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Authors:
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Li-Hong Zhang; San-Feng Chen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-6 |
Journal Detail:
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Title: World journal of microbiology & biotechnology Volume: - ISSN: 1573-0972 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9012472 Medline TA: World J Microbiol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China, zhangyongyuan0@163.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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