| Proximal tubular epithelial cells are generated by division of differentiated cells in the healthy kidney. | |
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MedLine Citation:
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PMID: 16987990 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We searched for evidence for a contribution of stem cells in growth of the proximal S3 segments of healthy rats. According to the stem cell model, stem cells are undifferentiated and slow cycling; the bulk of cycling cells are transit amplifying, rapidly cycling cells. We show the following. 1) By continuous application of a thymidine analog (ThA) for 7 days, S3 proximal epithelial cells in healthy kidneys display a high-cycling rate. 2) Slow-cycling cells, identified by lack of ThA uptake during 14 days of continuous ThA application up to death and by expression of the cell cycle protein Ki67 at death, have the same degree of differentiation as quiescent cells. 3) To detect rapidly cycling cells, rats were killed at various time points after injection of a ThA. Double immunofluorescence for ThA and a cell cycle marker was performed, with colocalization indicating successive divisions. During one week after division, daughter cells display a very low proliferation rate, indicating the absence of rapidly cycling cells. 4) Labeling with cyclin D1 showed that this low proliferation rate is due to cycle arrest. 5) More than 50% of the S3 cells entered the cell cycle 36 h after a potent proliferative stimulus (lead acetate injection). We conclude that generation of new cells in the proximal tubule relies on division of differentiated, normally slow-cycling cells. These may rapidly enter the cycle under an adequate stimulus. |
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Authors:
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Alexander Vogetseder; Thomas Palan; Desa Bacic; Brigitte Kaissling; Michel Le Hir |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-20 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 292 ISSN: 0363-6143 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-02-13 Completed Date: 2007-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C807-13 Citation Subset: IM |
Affiliation:
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Institute of Anatomy, Univ. of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Differentiation* Cell Lineage Cell Proliferation / drug effects Cells, Cultured Cyclin D1 / metabolism Deoxyuridine / analogs & derivatives, pharmacology Epithelial Cells / cytology, physiology* Fluorescent Antibody Technique Kidney Tubules, Proximal / cytology* Male Organometallic Compounds / pharmacology Rats Rats, Wistar Stem Cells / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Organometallic Compounds; 136601-57-5/Cyclin D1; 301-04-2/lead acetate; 50-90-8/5-chloro-2'-deoxyuridine; 951-78-0/Deoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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