Document Detail


Proximal colon distention increases Fos expression in the lumbosacral spinal cord and activates sacral parasympathetic NADPHd-positive neurons in rats.
MedLine Citation:
PMID:  9455894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fos expression induced by nociceptive mechanical distention of the proximal colon was examined in the lumbosacral spinal cord in freely moving rats equipped with a chronic balloon in the proximal colon. Fos protein in lumbosacral neurons was detected immunocytochemically, and colocalization with nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) activity was determined histochemically at 1 hour after distention. Distention of the proximal colon (10 ml, 30 seconds on/off for 10 minutes, about 90 mm Hg) increased the number of Fos-positive cells in the lumbar 6 (L6) and sacral 1 and 2 (S1, S2) segments, whereas no change was observed in the L1-L5 and S3 segments compared with the sham distended group or with animals that received no treatment. In L6-S2 segments, Fos-positive neurons were increased by two-fold in laminae I-VII (mainly in laminae I and outer II) and area X (surrounding the central canal) and by nine-fold in the sacral parasympathetic nucleus. Results of time course studies indicate that the maximal increase in Fos expression observed at 1 hour after distention returns to basal levels within 4 hours. In the S1 segment, distention of the proximal colon increased the percentage of NADPHd/Fos-positive neurons selectively in the parasympathetic nucleus by 40% compared with less than 4% in the sham distention group; the number and pattern of NADPHd-stained cells were not modified. These results indicate that noxious distention of the proximal colon for a short duration in awake rats selectively activates neurons in the L6-S2 segments of the dorsal horn mainly in laminae involved in nociceptive and autonomic processing. The marked activation of NADPHd-positive neurons in the sacral parasympathetic nucleus suggests a possible role of nitric oxide in the visceroautonomic reflexes induced by distention of the proximal colon.
Authors:
V Martínez; L Wang; E Mayer; Y Taché
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  390     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-03-10     Completed Date:  1998-03-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  311-21     Citation Subset:  IM    
Affiliation:
Department of Medicine, CURE: Digestive Diseases Research Center, West Los Angeles VA Medical Center, California 90073, USA. ytache@ucla.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Balloon Dilatation
Colon / metabolism*
Histocytochemistry
Immunohistochemistry
Lumbosacral Region
Male
NADPH Dehydrogenase / analysis*
Neurons / enzymology*
Parasympathetic Nervous System / cytology,  enzymology*
Proto-Oncogene Proteins c-fos / analysis*
Rats
Rats, Sprague-Dawley
Sacrococcygeal Region
Spinal Cord / metabolism*
Grant Support
ID/Acronym/Agency:
DK-41302/DK/NIDDK NIH HHS; K05 MH-00663/MH/NIMH NIH HHS; R01 DK-33061/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; EC 1.6.99.1/NADPH Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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