Document Detail


Proving the antimicrobial spectrum of an amphoteric surfactant-sol-gel coating: a food-borne pathogen study.
MedLine Citation:
PMID:  18536948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An antimicrobial coating was evaluated in this work for its antimicrobial efficacy against common food-borne pathogens. Dodecyl-di(aminoethyl)-glycine, an organic disinfectant, was immobilized in a silicon oxide matrix to generate thin films over surfaces by means of the sol-gel process. Tetraethoxysilane was used as the polymeric precursor. No alteration of optical transparency on the covered surfaces was observed. Topographic images obtained with atomic force microscopy showed a homogeneous film with no additional roughness added by the polymer to the surface. The attenuated total reflectance-Fourier transform infrared spectral data showed the presence of dodecyl-di(aminoethyl)-glycine in the silicon oxide network after a normal cleaning procedure. The antimicrobial efficacy test was performed by exposing coated slides to suspensions of common food-borne pathogens: Escherichia coli, Staphyloccocus aureus, E. coli O157:H7, Salmonella typhi, S. cholerasuiss, Listeria innocua and L. monocytogenes. The coating activity was not only bacteriostatic but also bactericidal. The percent reduction of viable microorganism exposure over 24 h to the coated surface ranged between 99.5%, for the more resistant gram-positive bacteria, and over 99.999%, for most gram-negative bacteria. The silicon matrix itself did not account for any reduction of viable microbial, even more an increase was observed.
Authors:
G J Copello; S Teves; J Degrossi; M D'Aquino; M F Desimone; L E Díaz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-07
Journal Detail:
Title:  Journal of industrial microbiology & biotechnology     Volume:  35     ISSN:  1367-5435     ISO Abbreviation:  J. Ind. Microbiol. Biotechnol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705544     Medline TA:  J Ind Microbiol Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1041-6     Citation Subset:  IM    
Affiliation:
Cátedra de Química Analítica Instrumental, Universidad de Buenos Aires (UBA), Facultad de Farmacia y Bioquímica, Junín 956 - Piso 3 masculine (1113), Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents / chemistry,  pharmacology*
Bacteria / drug effects*
Colony Count, Microbial
Disinfectants / chemistry,  pharmacology*
Food Industry / methods
Food Microbiology*
Gels / chemistry,  pharmacology*
Glycine / analogs & derivatives*,  chemistry,  pharmacology
Microscopy, Atomic Force
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Surface-Active Agents / chemistry,  pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Disinfectants; 0/Gels; 0/Surface-Active Agents; 56-40-6/Glycine; 6843-97-6/dodicin

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