| Protoporphyrin IX distribution following local application of 5-aminolevulinic acid and its esterified derivatives in the tissue layers of the normal rat colon. | |
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MedLine Citation:
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PMID: 11720447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic diagnosis and especially therapy after sensitization with 5-aminolevulinic acid (ALA) is hampered by limitations of uptake and distribution of ALA due to its hydrophilic nature. Chemical modification of ALA into its more lipophilic esters seems to be promising to overcome these problems. The aim of the present study was to investigate the comparative kinetics of protoporphyrin IX (PPIX) fluorescence in rat colonic tissue after topical application of ALA and its esterified derivatives, ALA-hexylester (h-ALA), ALA-methylester (m-ALA) and ALA-benzylester (b-ALA). Fluorescence intensity induced by PPIX in normal colonic tissue was quantified using fluorescence microscopy at 1, 2, 4, 6 and 8 h after sensitization. Mucosa exhibited higher fluorescence levels compared to the underlying submucosa or smooth muscle. Peak fluorescence intensities were seen 4 h after local sensitization with 86.0 mol ml(-1) ALA (513 +/- 0.57 counts per pixel), 6.6 mol ml(-1) m-ALA (508 +/- 35.50) and 4.8 mol ml(-1) h-ALA (532 +/- 128.80), and 6 h after sensitization with 4.6 mol ml(-1) b-ALA (468 +/- 190.27). A 13-18 times lower concentration of ALA esters was required for fluorescence intensities reached with ALA alone. A similar degree of the fluorescence ratio between mucosa and muscularis (5-6:1) was detected for ALA and its derivatives. The time point of the maximum value of this ratio was consistent with peak fluorescence levels for ALA and each ALA-ester. The clinical feasibility and the advantages of topical ALA ester-based fluorescence for detection of malignant and premalignant lesions need further investigations. |
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Authors:
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E Endlicher; P Rümmele; F Hausmann; R Krieg; R Knüchel; H C Rath; J Schölmerich; H Messmann |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of cancer Volume: 85 ISSN: 0007-0920 ISO Abbreviation: Br. J. Cancer Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-26 Completed Date: 2002-02-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370635 Medline TA: Br J Cancer Country: Scotland |
Other Details:
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Languages: eng Pagination: 1572-6 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine I, University of Regensburg, 93042 Regensburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminolevulinic Acid
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administration & dosage,
analogs & derivatives*,
pharmacokinetics* Animals Colon / drug effects, metabolism* Female Kinetics Microscopy, Fluorescence Photosensitizing Agents / administration & dosage, pharmacokinetics* Protoporphyrins / analysis* Rats Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/5-aminolevulinic acid hexyl ester; 0/Photosensitizing Agents; 0/Protoporphyrins; 0/delta-aminolevulinic acid methyl ester; 106-60-5/Aminolevulinic Acid; 553-12-8/protoporphyrin IX |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Br J Cancer ISSN: 0007-0920 ISSN: 1532-1827 Publisher: Nature Publishing Group |
Article Information Download PDF  Copyright 2001, Cancer Research Campaign Received Day: 20 Month: 02 Year: 2001 Revision Received Day: 25 Month: 06 Year: 2001 Accepted Day: 19 Month: 07 Year: 2001 Print publication date: Month: 11 Year: 2001 Volume: 85 Issue: 10 First Page: 1572 Last Page: 1576 ID: 2363929 Publisher Item Identifier: 6692124 DOI: 10.1054/bjoc.2001.2124 PubMed Id: 11720447 |
| Protoporphyrin IX distribution following local application of 5-aminolevulinic acid and its esterified derivatives in the tissue layers of the normal rat colon | |
| E Endlicher1 | |
| P Rümmele2 | |
| F Hausmann3 | |
| R Krieg1 | |
| R Knüchel2 | |
| H C Rath1 | |
| J Schölmerich1 | |
| H Messmann1 | |
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1Department of Internal Medicine I, 2Institute of Pathology, 3Institute of Inorganic Chemistry, University of Regensburg, Regensburg, 93042, Germany |
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| *Author for correspondence: helmut.messman@klinik.uni-regensburg.de |
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Article Categories:
Keywords: 5-aminolevulinic acid, 5-aminolevulinic esters, protoporphyrin IX, fluorescence, colon. |
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