Document Detail

Protoplast formation of the coccolithophorid Pleurochrysis haptonemofera in hypoosmotic K+ solution: shedding of the coccosphere and regrowth of the protoplast in normal medium.
MedLine Citation:
PMID:  17131046     Owner:  NLM     Status:  MEDLINE    
Both coccolith-bearing cells (C-cells) and naked cells (N-cells) of the coccolithophorid Pleurochrysis haptonemofera can grow in salinities of more than 7 per thousand (about 20% of a "normal" sea water salinity [35 per thousand]), with the highest growth rates in salinities of more than 14 per thousand. Microscopic observations of cells suspended in 100 mM NaCl (7 per thousand) showed that, while N-cells were swelling uniformly all over the cell surface, C-cells were bulging the plasma membrane from the hole of the coccosphere at the apical (flagellar) pole of the cell. Effects of several cations and anions on the morphological change of C-cells under hypoosmotic pressure were investigated. When 100 mM K(+) was used, protoplasts were released from the coccosphere completely in almost all the cells. This phenomenon was shown with K(+) most effectively. The protoplasts could grow in the fresh medium and form the first coccolith within 9 h.
Takatoshi Takayanagi; Yasutaka Hirokawa; Maki Yamamoto; Toshichika Ohki; Shoko Fujiwara; Mikio Tsuzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-28
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  9     ISSN:  1436-2228     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-03-02     Completed Date:  2007-07-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  56-65     Citation Subset:  IM    
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
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MeSH Terms
Algae / cytology,  drug effects*,  growth & development,  physiology*
Cell Membrane / drug effects
Culture Media / chemistry
Osmotic Pressure
Potassium Chloride / pharmacology*
Protoplasts / physiology*
Seawater / chemistry*,  microbiology
Sodium Chloride / pharmacology
Time Factors
Reg. No./Substance:
0/Culture Media; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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