| Proton nuclear magnetic resonance spectroscopy of lactate production in isolated rat liver during cold preservation. | |
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MedLine Citation:
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PMID: 8674359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lactate-edited 1H NMR difference spectra have been acquired from intact rat liver tissue following flushing and preservation in ice. A peak, initially at 1.26 ppm, was seen to increase in the liver tissue with preservation time. This peak was assigned to lactate, despite the fact that its chemical shift was initially shifted by approximately -0.1 ppm relative to an externally added standard. The assignment was based on the following: (a) the peak increased over a 24-h ischemic storage period; (b) it was coupled to a signal 2.78 +/- 0.02 ppm upfield; and (c) a parallel increase in lactate was noted in perchloric acid extracts of tissue from the same liver. An additional peak, assigned to alanine, was also observed during storage and was also shifted by approximately -0.1 ppm. Inclusion of dimethyl sulfoxide, which readily permeates liver tissue, demonstrated that this chemical shift alteration was a tissue-specific effect. These results demonstrate that 1H NMR spectroscopy of intact liver tissue during hypothermic ischemia is possible, though chemical shift assignments should be made with caution. |
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Authors:
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C J Lockett; A L Busza; E Proctor; T A Churchill; S R Williams; B J Fuller |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryobiology Volume: 33 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-08-09 Completed Date: 1996-08-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 271-5 Citation Subset: IM |
Affiliation:
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Institute of Child Health, Royal College of Surgeons Unit of Biophysics, London, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cold Temperature Lactates / biosynthesis* Lactic Acid Liver* / metabolism* Magnetic Resonance Spectroscopy Male Organ Preservation / methods* Rats Rats, Wistar Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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