Document Detail


Proton magnetic resonance study of cycloheximide-ribosome interactions.
MedLine Citation:
PMID:  2206134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cycloheximide-ribosome interactions from sensitive and resistant organisms were studied by proton magnetic resonance spectroscopic techniques. The two methyl resonances of cycloheximide upon interaction with ribosomes from Saccharomyces cerevisiae showed preferential broadening. Comparison of cycloheximide line broadening as effected by ribosomes from S. cerevisiae (sensitive) and Microsporum canis (resistant) revealed that less cycloheximide is bound to the M. canis ribosomes. From the decrease in line broadening observed with increasing temperature it may be concluded that cycloheximide-ribosome interaction is a fast exchange reaction. Tetracycline did not compete with cycloheximide for binding site(s) on the ribosomes of S. cerevisiae.
Authors:
V Mandiyan; S Devarajan; G R Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry international     Volume:  21     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1990  
Date Detail:
Created Date:  1990-10-19     Completed Date:  1990-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
Affiliation:
Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore.
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cycloheximide / metabolism*,  pharmacology
Drug Resistance, Microbial
Kinetics
Magnetic Resonance Spectroscopy
Microsporum / drug effects,  metabolism*
Molecular Structure
Ribosomes / metabolism*
Saccharomyces cerevisiae / drug effects,  metabolism*
Temperature
Tetracycline / pharmacology
Chemical
Reg. No./Substance:
60-54-8/Tetracycline; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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