Document Detail


Proton leak and control of oxidative phosphorylation in perfused, resting rat skeletal muscle.
MedLine Citation:
PMID:  8764890     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distribution of control over the resting respiration rate (and other variables) was assessed in perfused rat skeletal muscle. The results indicate that the general pattern of control in resting muscle is similar to that seen in isolated rat liver cells (Brown et al. (1990) Biochem. J. 192, 355-362). Control over resting mitochondrial oxygen consumption was distributed between reactions involved in substrate oxidation (flux control coefficient = 0.44 +/- 0.28) and those involved in ATP turnover (0.21 +/- 0.09). The mitochondrial proton leak also had an important share of the control (0.38 +/- 0.21). Since proton leak also has significant control over the resting respiration rate of isolated liver cells and liver and skeletal muscle account for around 60% of the standard metabolic rate of a rat, then these results indicate the potential importance of proton leak as a mechanism of regulating metabolic rate.
Authors:
D F Rolfe; M D Brand
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1276     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-19     Completed Date:  1996-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Cambridge, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Kinetics
Mitochondria, Muscle / metabolism
Muscle, Skeletal / metabolism*
Oxidative Phosphorylation
Oxygen Consumption
Perfusion
Protons
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Protons

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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