| Proton gradient-dependent transport of glycine in rabbit renal brush-border membrane vesicles. | |
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MedLine Citation:
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PMID: 2822708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study describes evidence for the existence of a H+/glycine symport system in rabbit renal brush-border membrane vesicles. An inward proton gradient stimulates glycine transport across the brush-border membrane, and this H+-driven glycine uptake is attenuated by the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone. It is a positive rheogenic process, i.e. the H+-dependent glycine uptake is further enhanced by an intravesicular negative potential. Glycine uptake is stimulated to a lesser degree by an inward Na+ gradient. H+-dependent glycine uptake is inhibited by sarcosine (69%), an analog amino acid, imino acids (proline 81%, hydroxy proline 67%), and beta-alanine (31%), but not by neutral (L-leucine) or basic (L-lysine) amino acids. The results demonstrate that H+ glycine co-transport system in rabbit renal brush-border membrane vesicles is a carrier-mediated electrogenic process and that transport is shared by imino acids and partially by beta-alanine. |
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Authors:
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V M Rajendran; J A Barry; J G Kleinman; K Ramaswamy |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 262 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1987 Nov |
Date Detail:
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Created Date: 1987-12-10 Completed Date: 1987-12-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 14974-7 Citation Subset: IM |
Affiliation:
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Department of Medicine, Veterans Administration Medical Center, Milwaukee, Wisconsin. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Animals Biological Transport / drug effects Carbon Radioisotopes Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology Female Glycine / metabolism* Kidney Cortex / metabolism* Kinetics Microvilli / metabolism* Protons Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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AM 33849/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Radioisotopes; 0/Protons; 370-86-5/Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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