| Proton-Resistant Quantum Dots: Stability in Gastrointestinal Fluids and Implications for Oral Delivery of Nanoparticle Agents. | |
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MedLine Citation:
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PMID: 20379372 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Semiconductor quantum dots (QDs) have shown great promise as fluorescent probes for molecular, cellular and in-vivo imaging. However, the fluorescence of traditional polymer-encapsulated QDs is often quenched by proton-induced etching in acidic environments. This is a major problem for QD applications in the gastrointestinal tract because the gastric (stomach) environment is strongly acidic (pH 1-2). Here we report the use of proton-resistant surface coatings to stabilize QD fluorescence under acidic conditions. Using both hyperbranched polyethylenimine (PEI) and its polyethylene glycol derivative (PEG grafted PEI), we show that the fluorescence of core-shell CdSe/CdS/ZnS QDs is effectively protected from quenching in simulated gastric fluids. In comparison, amphiphilic lipid or polymer coatings provide no protection under similarly acidic conditions. The proton-resistant QDs are found to cause moderate membrane damage to cultured epithelial cells, but PEGylation (PEG grafting) can be used to reduce cellular toxicity and to improved nanoparticle stability. |
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Authors:
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Aaron M Mohs; Hongwei Duan; Brad A Kairdolf; Andrew M Smith; Shuming Nie |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Nano research Volume: 2 ISSN: 1998-0000 ISO Abbreviation: - Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2010-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101518856 Medline TA: Nano Res Country: - |
Other Details:
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Languages: ENG Pagination: 500-508 Citation Subset: - |
Affiliation:
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Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 101 Woodruff Circle NE, Suite 2001, Atlanta, Georgia 30322, USA. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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P20 GM072069-01//NIGMS NIH HHS; R01 CA108468-01//NCI NIH HHS; U01 HL080711-01//NHLBI NIH HHS; U54 CA119338-01//NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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