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Protocol for Ex Vivo Incubation of Drosophila Primary Post-embryonic Haemocytes for Real-Time Analyses.
MedLine Citation:
PMID:  22144286     Owner:  NLM     Status:  In-Data-Review    
The cellular branch of the Drosophila larval innate immune system consists of three immunosurveillance (haemocyte) cell types: plasmatocytes, crystal cells, and lamellocytes. In order to examine haemocyte cytoskeletal dynamics or migration, most researchers use embryos or in vitro cell culture systems, but very little is known about the behaviour of post-embryonic haemocytes. The current method employs an ex vivo system, in which post-embryonic haemocytes are isolated for short-term analysis, in order to investigate various aspects of their behaviour during events requiring cytoskeleton dynamics and Rho GTPase signalling.
Christopher J Sampson; Michael J Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  827     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  359-67     Citation Subset:  IM    
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UK.
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