| Protochlorophyllide b does not occur in barley etioplasts. | |
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MedLine Citation:
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PMID: 10094504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Barley (Hordeum vulgare L.) etioplasts were isolated, and the pigments were extracted with acetone. The extract was analyzed by HPLC. Only protochlorophyllide a and no protochlorophyllide b was detected (limit of detection < 1% of protochlorophyllide a). Protochlorophyllide b was synthesized starting from chlorophyll b and incubated with etioplast membranes and NADPH. In the light, photoconversion to chlorophyllide b was observed, apparently catalyzed by NADPH :protochlorophyllide oxidoreductase. In darkness, reduction of the analogue zinc protopheophorbide b to zinc 7-hydroxy-protopheophorbide a was observed, apparently catalyzed by chlorophyll b reductase. We conclude that protochlorophyllide b does not occur in detectable amounts in etioplasts, and even traces of it as the free pigment are metabolically unstable. Thus the direct experimental evidence contradicts the idea by Reinbothe et al. (Nature 397 (1999) 80-84) of a protochlorophyllide b-containing light-harvesting complex in barley etioplasts. |
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Authors:
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V Scheumann; H Klement; M Helfrich; U Oster; S Schoch; W Rüdiger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 445 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-04-20 Completed Date: 1999-04-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 445-8 Citation Subset: IM |
Affiliation:
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Botanisches Institut der Ludwig-Maximilians-Universität München, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Hordeum
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chemistry* Molecular Structure Protochlorophyllide / analysis* |
| Chemical | |
Reg. No./Substance:
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20369-67-9/Protochlorophyllide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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