Document Detail


Protocell-like microspheres from thermal polyaspartic acid.
MedLine Citation:
PMID:  17120121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the most prominent amino acids to appear in monomer-generating origin-of-life experiments is aspartic acid. Hugo Schiff found in 1897 that aspartic acid polymerizes when heated to form polyaspartylimide which hydrolyzes in basic aqueous solution to form thermal polyaspartic acid which is a branched polypeptide. We recently reported at the ISSOL 2005 Conference that commercially made thermal polyaspartic acid forms microspheres when heated in boiling water and allowed to cool. In a new experiment we heated aspartic acid at 180 degrees C for up to 100 h to form thermal polyaspartylimide which when heated in boiling water without addition of base hydrolyzed to form thermal polyaspartic acid which upon cooling formed microspheres. Thermal polyaspartic acid microspheres appear protocell-like in the sense of being prebiotically plausible lattices or containers that could eventually have been filled with just the right additions of primordial proteins, nucleic acids, lipids, and metabolites so as to constitute protocells capable of undergoing further chemical and biological evolution. Thermal polyaspartic acid microspheres are extremely simple models of protocells that are more amenable to precise quantitative experimental investigation than the proteinoid microspheres of Sidney W. Fox. We present here scanning electron microscope images of such thermal polyaspartic acid microspheres. Figure 1 shows thermal polyaspartic acid microspheres from L: -aspartic acid heated at 180 degrees C for 50 h, at a magnification of 3,500x. Figure 2 shows thermal polyaspartic acid microspheres from the same sample at a magnification of 7,000x. The thermal polyaspartic acid microspheres have a diameter of approximately 1 mum These images were viewed with a Hitachi S2460N scanning electron microscope at 20 kV acceleration voltage. Figure 1 Thermal polyaspartic acid microspheres from L: -aspartic acid heated at 180 degrees C for 50 h, at a magnification of 3,500x. Figure 2 Thermal polyaspartic acid microspheres from L: -aspartic acid heated at 180 degrees C for 50 h, at a magnification of 7,000x.
Authors:
Peter R Bahn; Aristotel Pappelis; John Bozzola
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life     Volume:  36     ISSN:  0169-6149     ISO Abbreviation:  Orig Life Evol Biosph     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-01-19     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610391     Medline TA:  Orig Life Evol Biosph     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  617-9     Citation Subset:  IM; S    
Affiliation:
Bahn Biotechnology Co., 10415 E. Boyd Road, Mt. Vernon, IL 62864, USA. pbahn@mvn.net
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MeSH Terms
Descriptor/Qualifier:
Biogenesis
Evolution, Chemical
Microscopy, Electron, Scanning
Microspheres*
Peptides / chemistry*
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
0/Peptides; 26063-13-8/polyaspartate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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