Document Detail

Protocatechuic acid from Alpinia oxyphylla against MPP+-induced neurotoxicity in PC12 cells.
MedLine Citation:
PMID:  16223555     Owner:  NLM     Status:  MEDLINE    
An ethyl acetate extract of Alpinia oxyphylla was found to possess neuroprotective activity against 1-methyl-4-phenylpyridinium ion (MPP(+)) induced apotosis and oxidative stress in cultured PC12 cells. From the extract, a phenolic compound was isolated through bioassay-guided fractionation and identified as protocatechuic acid (PCA) by IR, MS, and (1)H and (13)C NMR spectroscopy. It was the first time which was isolated from the kernels of A. oxyphylla. Exposure of PC12 cells to 1mM MPP(+) may cause significant viability loss and apoptotic cell death. PCA stimulated PC12 cellular proliferation and markedly attenuated MPP(+)-induced apoptotic cell death in a dose-dependent manner. By observing the nuclear morphological changes and flow cytometric analysis, PCA showed its significant effect on protecting PC12 cells against MPP(+)-induced apoptosis. Meanwhile, PCA enhanced the activities of superoxide dismutase (SOD) and catalase (CAT) in PC12 cells. In addition, PCA also dose-dependently reduced the hydrogen peroxide (H(2)O(2))- or sodium nitroprusside (SNP)-induced cell death in PC12 cells. The results suggest that PCA may be one of the primary active components in the kernels of A. oxyphylla and provide a useful therapeutic strategy for the treatment of oxidative stress-induced neurodegenerative disease such as Parkinson's disease.
L J An; S Guan; G F Shi; Y M Bao; Y L Duan; B Jiang
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Publication Detail:
Type:  Journal Article     Date:  2005-10-11
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  44     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2005-12-16     Completed Date:  2006-04-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  436-43     Citation Subset:  IM    
Department of Bioscience and Biotechnology, Dalian University of Technology, PR China.
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MeSH Terms
1-Methyl-4-phenylpyridinium / toxicity
Alpinia / chemistry*
Apoptosis / drug effects*
Catalase / metabolism
Cell Survival / drug effects*
Dose-Response Relationship, Drug
Flow Cytometry
Hydrogen Peroxide
Hydroxybenzoic Acids / chemistry,  isolation & purification,  pharmacology*
Neuroprotective Agents / pharmacology*
Oxidative Stress / drug effects
PC12 Cells
Plant Extracts / pharmacology
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Hydroxybenzoic Acids; 0/Neuroprotective Agents; 0/Plant Extracts; 48134-75-4/1-Methyl-4-phenylpyridinium; 7722-84-1/Hydrogen Peroxide; 99-50-3/protocatechuic acid; EC; EC Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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