| Protocatechuic acid from Alpinia oxyphylla against MPP+-induced neurotoxicity in PC12 cells. | |
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MedLine Citation:
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PMID: 16223555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An ethyl acetate extract of Alpinia oxyphylla was found to possess neuroprotective activity against 1-methyl-4-phenylpyridinium ion (MPP(+)) induced apotosis and oxidative stress in cultured PC12 cells. From the extract, a phenolic compound was isolated through bioassay-guided fractionation and identified as protocatechuic acid (PCA) by IR, MS, and (1)H and (13)C NMR spectroscopy. It was the first time which was isolated from the kernels of A. oxyphylla. Exposure of PC12 cells to 1mM MPP(+) may cause significant viability loss and apoptotic cell death. PCA stimulated PC12 cellular proliferation and markedly attenuated MPP(+)-induced apoptotic cell death in a dose-dependent manner. By observing the nuclear morphological changes and flow cytometric analysis, PCA showed its significant effect on protecting PC12 cells against MPP(+)-induced apoptosis. Meanwhile, PCA enhanced the activities of superoxide dismutase (SOD) and catalase (CAT) in PC12 cells. In addition, PCA also dose-dependently reduced the hydrogen peroxide (H(2)O(2))- or sodium nitroprusside (SNP)-induced cell death in PC12 cells. The results suggest that PCA may be one of the primary active components in the kernels of A. oxyphylla and provide a useful therapeutic strategy for the treatment of oxidative stress-induced neurodegenerative disease such as Parkinson's disease. |
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Authors:
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L J An; S Guan; G F Shi; Y M Bao; Y L Duan; B Jiang |
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Publication Detail:
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Type: Journal Article Date: 2005-10-11 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 44 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2005-12-16 Completed Date: 2006-04-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 436-43 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, Dalian University of Technology, PR China. guanshui_0518@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-4-phenylpyridinium
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toxicity Alpinia / chemistry* Animals Apoptosis / drug effects* Catalase / metabolism Cell Survival / drug effects* Dose-Response Relationship, Drug Flow Cytometry Hydrogen Peroxide Hydroxybenzoic Acids / chemistry, isolation & purification, pharmacology* Neuroprotective Agents / pharmacology* Oxidative Stress / drug effects PC12 Cells Plant Extracts / pharmacology Rats Superoxide Dismutase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Hydroxybenzoic Acids; 0/Neuroprotective Agents; 0/Plant Extracts; 48134-75-4/1-Methyl-4-phenylpyridinium; 7722-84-1/Hydrogen Peroxide; 99-50-3/protocatechuic acid; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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