Document Detail


Proto-oncogene expression in porcine myocardium subjected to ischemia and reperfusion.
MedLine Citation:
PMID:  1385005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular basis of myocardial adaptation to ischemia and reperfusion is poorly understood. It is thought that nuclear proto-oncogenes act as third messengers, converting cytoplasmic signal transduction into long-term changes of gene expression. We studied the expression of six nuclear proto-oncogenes (Egr-1, c-fos, fosB, c-jun, junB, and c-myc) in myocardium subjected to ischemia and reperfusion in anesthetized pigs. Stunning was achieved by two 10-minute left anterior descending coronary artery occlusions separated by 30 minutes of reperfusion. Hearts were excised after the first occlusion, after the first reperfusion, and at 30, 120, 150, and 210 minutes of reperfusion after the second occlusion. Total RNA was prepared from stunned as well as normally perfused myocardial tissue and subjected to Northern blotting. The response of the six nuclear proto-oncogenes varied.fosB gene expression was never detected. The c-myc gene was expressed, but its level was unchanged by ischemia. c-jun expression was slightly increased by ischemia (3.1 +/- 0.6-fold). The c-fos, Egr-1, and junB genes were highly induced, being fivefold to sevenfold higher in experimental than in control tissue. In three animals pretreated with the beta 1-antagonist metoprolol and then subjected to the above experimental protocol, the induction of proto-oncogenes was similar to that in nonblocked controls. Our results show that the myocardial adaptive response to ischemic stress includes the induction of at least four transcription factors that may be further operative in repair processes and angiogenesis.
Authors:
T Brand; H S Sharma; K E Fleischmann; D J Duncker; E O McFalls; P D Verdouw; W Schaper
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Circulation research     Volume:  71     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1992-12-18     Completed Date:  1992-12-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1351-60     Citation Subset:  IM    
Affiliation:
Department of Experimental Cardiology, Max Planck Institute of Physiological and Clinical Research, Bad Neuheim, FRG.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Gene Expression*
Genes, fos
Genes, jun
Genes, myc
Heart / drug effects,  physiopathology*
Hemodynamics
Metoprolol / pharmacology
Proto-Oncogenes / genetics*
RNA / isolation & purification
Reperfusion Injury / genetics*,  physiopathology
Swine
Chemical
Reg. No./Substance:
37350-58-6/Metoprolol; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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