Document Detail

Proto-oncogene expression in avian hematopoietic tissues.
MedLine Citation:
PMID:  1886713     Owner:  NLM     Status:  MEDLINE    
Previous findings from this laboratory (Kim & Baluda, 1988) have shown that the proto-oncogenes ETS, FPS, MHT (RAF), MYC and REL are expressed in avian myeloblastosis virus (AMV)-transformed cells, whereas the MYB gene is repressed. In this study five different chicken hematopoietic tissues which contained varying concentrations of target cells for AMV transformation were analyzed to determine whether the expression of these proto-oncogenes resulted from, or was altered by, v-myb-induced leukemogenesis. Poly-A+ RNA from hematopoietic cells of 11-13 day yolk sac, 16 day embryonic spleen, 1 day post-hatch bursa of Fabricius, bone marrow and thymus, as well as from chicken embryonic fibroblasts (CEF) was examined by Northern blot analysis. All five proto-oncogenes were found to be expressed in the normal hematopoietic tissues. The ETS, MHT (RAF), MYC, and REL genes, but not FPS, were expressed in CEF. The expression of these five proto-oncogenes was not quantitatively or qualitatively altered in AMV-transformed myeloid cells as compared with their normal counterparts. While their expression is part of the hematopoietic phenotype of the target cells and as such is necessary for susceptibility to AMV transformation, it is not sufficient because thymocytes with a high level of expression are not transformed. This is in contrast to MYB expression, which is totally repressed in leukemic cells but probably not as a result of v-myb expression.
E R Schuur; M A Baluda
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  6     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-10-04     Completed Date:  1991-10-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1409-15     Citation Subset:  IM    
Department of Pathology, UCLA School of Medicine 90024.
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MeSH Terms
Avian myeloblastosis virus / isolation & purification
Blotting, Northern
Bone Marrow / metabolism
Bone Marrow Cells
Bursa of Fabricius / cytology,  metabolism
Cell Transformation, Viral / genetics,  physiology
Cells, Cultured
Chick Embryo
Fibroblasts / cytology,  metabolism
Gene Expression*
Hematopoietic System / metabolism*
Protein-Tyrosine Kinases
Proto-Oncogene Proteins / genetics,  metabolism
Proto-Oncogene Proteins c-ets
Proto-Oncogene Proteins c-myc / genetics,  metabolism
Proto-Oncogene Proteins c-raf
Proto-Oncogene Proteins c-rel
Proto-Oncogenes / genetics*
Spleen / cytology,  metabolism
Thymus Gland / cytology,  metabolism
Transcription Factors*
Grant Support
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-ets; 0/Proto-Oncogene Proteins c-myc; 0/Proto-Oncogene Proteins c-rel; 0/Transcription Factors; 110736-90-8/proto-oncogene protein c-fes-fps; EC Kinases; EC Proteins c-raf

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