| Prothrombin biosynthesis: characterization of processing events in rat liver microsomes. | |
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MedLine Citation:
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PMID: 4052374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasma and hepatic microsomal forms of rat prothrombin have been compared by sodium dodecyl sulfate-polyacrylamide electrophoresis and isoelectric focusing. The major prothrombin species that accumulated in the microsomes of rats treated with warfarin had a molecular weight of 78 500 and a pI in 8 M urea of 6.3-6.5. Plasma prothrombin had a molecular weight of 83 500 and a pI of 5.3-5.7. Microsomes from normal rat liver contain a second pool of precursor with a molecular weight of 83 500, and digestion with the glycosidase Endo H indicated that this form has been processed to contain complex carbohydrates, while the Mr 78 500 form is a high mannose form and is the substrate for the vitamin K dependent carboxylase. Treatment of rats with tunicamycin revealed that glycosylation was not essential for carboxylation or secretion from the liver. Comparison of the aglyco forms of prothrombin and its precursors suggests that the intracellular forms contain a basic, Mr approximately 1500 peptide that is missing from the plasma form of prothrombin. |
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Authors:
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J C Swanson; J W Suttie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 24 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1985 Jul |
Date Detail:
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Created Date: 1985-12-05 Completed Date: 1985-12-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3890-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Male Microsomes, Liver / drug effects, enzymology* Molecular Weight Prothrombin / biosynthesis*, isolation & purification Rats Tunicamycin / pharmacology Vitamin K Deficiency / enzymology Warfarin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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AM-14881/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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11089-65-9/Tunicamycin; 81-81-2/Warfarin; 9001-26-7/Prothrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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