Document Detail

Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in nasopharyngeal carcinoma CNE2 cells.
MedLine Citation:
PMID:  15685366     Owner:  NLM     Status:  MEDLINE    
Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. However, the mechanism of the TPA effect on NPC remains unclear. In the present study, cells from a poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were stimulated by TPA and proteomics technology was carried out to find protein discrepancies between control and TPA-treated cells. Results revealed that TPA treatment in CNE2 cells could upregulate the expression of "triosephosphate isomerase" and "14-3-3 protein sigma" and downregulate the expression of "reticulocalbin 1 precursor", "nucleophosmin", "mitochondrial matrix protein p1 precursor", and "stathmin". The changes in the expression of these genes suggested that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on nasopharyngeal carcinoma cells were distinct from the effects on primary epithelial cells and we suggest reasons for these differences.
Pei-Zhou Jiang; Ming Gan; Hua Huang; Xin-Ming Shen; Shuang Wang; Kai-Tai Yao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  37     ISSN:  1672-9145     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-01     Completed Date:  2005-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-106     Citation Subset:  IM    
Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
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MeSH Terms
Annexin A5
Cell Line, Tumor
Flow Cytometry
Gene Expression Regulation, Neoplastic / drug effects*
Nasopharyngeal Neoplasms / genetics,  metabolism*
Peptide Mapping
Proteins / analysis*,  genetics
Proteomics / instrumentation,  methods*
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tetradecanoylphorbol Acetate / analogs & derivatives*,  pharmacology*
Reg. No./Substance:
0/Annexin A5; 0/Proteins; 16561-29-8/Tetradecanoylphorbol Acetate; 64604-09-7/12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate

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