Document Detail


Proteomics as a tool to investigate cell models for dopamine toxicity.
MedLine Citation:
PMID:  18585082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dopaminergic neurons are particularly exposed to oxidative stress because dopamine metabolism gives rise to endogenous toxins. Here, two different models for dopamine toxicity have been investigated using proteomics: stable alpha-synuclein-expressing neuroblastoma (SH-SY5Y) cells to understand how alpha-synuclein modulates dopamine toxicity at the central level, and cultured T-cell leukaemia (Jurkat) cells challenged with dopamine or l-dopa to evaluate protein changes on the surface of peripheral cells. alpha-synuclein-expressing cells were more resistant compared with wild-type cells, showing upregulation of antioxidant proteins including the Parkinson's disease-related DJ-1 protein. In addition, treatment of prototypical T-cells with l-dopa induced significant changes in proteins on the cell surface, indicating that peripheral blood lymphocytes may be good indicators of dopamine toxicity.
Authors:
Mauro Fasano; Tiziana Alberio; Monica Colapinto; Silvia Mila; Leonardo Lopiano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2008-06-27
Journal Detail:
Title:  Parkinsonism & related disorders     Volume:  14 Suppl 2     ISSN:  1353-8020     ISO Abbreviation:  Parkinsonism Relat. Disord.     Publication Date:  2008  
Date Detail:
Created Date:  2008-07-21     Completed Date:  2008-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513583     Medline TA:  Parkinsonism Relat Disord     Country:  England    
Other Details:
Languages:  eng     Pagination:  S135-8     Citation Subset:  IM    
Affiliation:
Department of Structural and Functional Biology, University of Insubria, Via Alberto da Giussano 12, Busto Arsizio (VA), Italy. mauro.fasano@uninsubria.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Dopamine / toxicity*
Humans
Neuroblastoma / pathology
Neurotoxicity Syndromes* / etiology,  metabolism,  pathology
Proteomics / methods*

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