| A Proteomics Search Algorithm Specifically Designed for High-Resolution Tandem Mass Spectra. | |
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MedLine Citation:
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PMID: 23323968 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The acquisition of high-resolution tandem mass spectra (MS/MS) is becoming more prevalent in proteomics, but most researchers employ peptide identification algorithms that were designed prior to this development. Here we demonstrate new software, Morpheus, designed specifically for high-mass accuracy data, based on a simple score that is little more than the number of matching products. For a diverse collection of datasets from a variety of organisms (E. coli, yeast, human) acquired on a variety of instruments (quadrupole-time of flight, ion trap-orbitrap, quadrupole-orbitrap) in different laboratories, Morpheus gives more spectrum, peptide, and protein identifications at a 1% false discovery rate (FDR) than Mascot, Open Mass Spectrometry Search Algorithm (OMSSA), and Sequest. Additionally, Morpheus is 1.5 to 4.6 times faster-depending on the dataset-than the next fastest algorithm, OMSSA. Morpheus was developed in C# .NET and is available free and open source under a permissive license. |
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Authors:
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Craig D Wenger; Joshua J Coon |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-17 |
Journal Detail:
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Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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