Document Detail

A proteomics search algorithm specifically designed for high-resolution tandem mass spectra.
MedLine Citation:
PMID:  23323968     Owner:  NLM     Status:  MEDLINE    
The acquisition of high-resolution tandem mass spectra (MS/MS) is becoming more prevalent in proteomics, but most researchers employ peptide identification algorithms that were designed prior to this development. Here, we demonstrate new software, Morpheus, designed specifically for high-mass accuracy data, based on a simple score that is little more than the number of matching products. For a diverse collection of data sets from a variety of organisms (E. coli, yeast, human) acquired on a variety of instruments (quadrupole-time-of-flight, ion trap-orbitrap, and quadrupole-orbitrap) in different laboratories, Morpheus gives more spectrum, peptide, and protein identifications at a 1% false discovery rate (FDR) than Mascot, Open Mass Spectrometry Search Algorithm (OMSSA), and Sequest. Additionally, Morpheus is 1.5 to 4.6 times faster, depending on the data set, than the next fastest algorithm, OMSSA. Morpheus was developed in C# .NET and is available free and open source under a permissive license.
Craig D Wenger; Joshua J Coon
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-31
Journal Detail:
Title:  Journal of proteome research     Volume:  12     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-01     Completed Date:  2013-08-29     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1377-86     Citation Subset:  IM    
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MeSH Terms
Chromatography, Liquid
Escherichia coli Proteins / isolation & purification,  metabolism
Saccharomyces cerevisiae Proteins / isolation & purification,  metabolism
Tandem Mass Spectrometry / methods*
Grant Support
Reg. No./Substance:
0/Escherichia coli Proteins; 0/Saccharomyces cerevisiae Proteins

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