| Proteomics of Pyrococcus Furiosus (Pfu): Identification of Extracted Proteins by Three Independent Methods. | |
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MedLine Citation:
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PMID: 23298259 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Pyrococcus Furiosus (Pfu) can used to be an excellent organism as a standard in proteomics because of its moderately sized genome and very little sequence duplication within the genome. We demonstrated a stable and consistent method to prepare proteins in bulk that eliminates growth and preparation as a source of uncertainty in the standard. We performed the proteomics workflows in different individual laboratories, using their own technology set up, as well as separated and integrated data analysis, demonstrated that Pfu as a suitable complex protein standard can not only be used to validate proteomic methods, work flows, and benchmark new instruments, but also facilitate comparison of experimental data generated over time, across instruments or labs. |
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Authors:
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Catherine C L Wong; Daniel Cociorva; Christine A Miller; Alexander Schmidt; Craig Monell; Ruedi Aebersold; John R Yates |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-8 |
Journal Detail:
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Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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