Document Detail

Proteomics of Pyrococcus furiosus (Pfu): Identification of Extracted Proteins by Three Independent Methods.
MedLine Citation:
PMID:  23298259     Owner:  NLM     Status:  MEDLINE    
Pyrococcus furiosus (Pfu) is an excellent organism to generate reference samples for proteomics laboratories because of its moderately sized genome and very little sequence duplication within the genome. We demonstrated a stable and consistent method to prepare proteins in bulk that eliminates growth and preparation as a source of uncertainty in the standard. We performed several proteomic studies in different laboratories using each laboratory's specific workflow as well as separate and integrated data analysis. This study demonstrated that a Pfu whole cell lysate provides suitable protein sample complexity to not only validate proteomic methods, work flows, and benchmark new instruments but also to facilitate comparison of experimental data generated over time and across instruments or laboratories.
Catherine C L Wong; Daniel Cociorva; Christine A Miller; Alexander Schmidt; Craig Monell; Ruedi Aebersold; John R Yates
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-18
Journal Detail:
Title:  Journal of proteome research     Volume:  12     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-01     Completed Date:  2013-07-05     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  763-70     Citation Subset:  IM    
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MeSH Terms
Archaeal Proteins / isolation & purification*
Chromatography, Liquid
Genome Size
Genome, Archaeal*
Liquid Phase Microextraction / methods
Mass Spectrometry
Observer Variation
Peptide Fragments / isolation & purification*
Pyrococcus furiosus / chemistry*,  genetics*
Reproducibility of Results
Grant Support
GM103533/GM/NIGMS NIH HHS; P01 AG031097/AG/NIA NIH HHS; P01 AG031097-03/AG/NIA NIH HHS; P41 RR011823/RR/NCRR NIH HHS; P41 RR011823/RR/NCRR NIH HHS; R01 HL079442/HL/NHLBI NIH HHS; R01 HL079442-07/HL/NHLBI NIH HHS; R01 MH067880/MH/NIMH NIH HHS; R01 MH067880-09/MH/NIMH NIH HHS
Reg. No./Substance:
0/Archaeal Proteins; 0/Peptide Fragments

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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