Document Detail


Proteomic studies of macrophage-derived foam cell from human U937 cell line using two-dimensional gel electrophoresis and tandem mass spectrometry.
MedLine Citation:
PMID:  14639101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors have identified 37 proteins in the whole-cell extracts from human monoblastic leukemia (U937) cell and macrophage-derived foam cell. The in vitro foam cell model was established by incubating the human U937 cells with oxidized low-density lipoprotein. The global changes in protein expressions between U937 foam cell and normal U937 cells were measured with two-dimensional polyacrylamide gel electrophoresis, and some proteins were trypsin-digested and then identified through tandem mass spectrometry after capillary liquid chromatography separation. Some of the identified proteins were validated via Internet links to the U937 proteomic map provided on the Expasy proteomics server. The experimental data can provide potential markers for atherosclerotic studies.
Authors:
Yan-Ling Yu; Zhen-Yu Huang; Peng-Yuan Yang; Yao-Cheng Rui; Peng-Yuan Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  42     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-25     Completed Date:  2004-03-05     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  782-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Fudan University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Gel, Two-Dimensional
Foam Cells / metabolism*
Humans
Mass Spectrometry
Peptides* / analysis,  isolation & purification
Proteomics / methods*
U937 Cells
Chemical
Reg. No./Substance:
0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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