Document Detail

Proteomic profiling of the acid tolerance response (ATR) during the enhanced biomethanation process from Taihu Blue Algae with butyrate stress on anaerobic sludge.
MedLine Citation:
PMID:  22921126     Owner:  NLM     Status:  Publisher    
Enhanced biomethanation with acid stress on anaerobic sludge, dehydrogenase activity, protein expression, and the primary proteomic profiling of microbial communities during the enhanced anaerobic digestion process from Taihu Blue Algae were investigated. It was found that the accumulation of organic acids and the specific biogas accumulation rate were 1.8 and 1.3 times of the control, when 10g/L and 7.5g/L of butyrate were selected for acid stress, respectively. Meanwhile, dehydrogenase activity of the 7.5g/L acid stress group exhibited an increase of 1.6 times of the control, and protein expression was also found to be enhanced sharply as revealed by 1D-PAGE. Finally, twenty of the matched protein spots through 2D-PAGE from both the control and the 7.5g/L stress groups were identified by MALDI-TOF MS, and five of which were proved to be involved in bioenergy metabolism. Significantly, ATR related proteins might be induced as the pIs of which were acidic as 5.92, 5.51 and 5.54, respectively.
Qun Yan; Yongcan Li; Bin Huang; Aijie Wang; Hua Zou; Hengfeng Miao; Rongqing Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-10
Journal Detail:
Title:  Journal of hazardous materials     Volume:  -     ISSN:  1873-3336     ISO Abbreviation:  J. Hazard. Mater.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422688     Medline TA:  J Hazard Mater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian 223300, China.
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