Document Detail


Proteomic identification of responsive proteins of Vibrio parahaemolyticus under high hydrostatic pressure (HHP).
MedLine Citation:
PMID:  24473993     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
High hydrostatic pressure processing (HHP) is currently being used as a treatment for certain foods to inhibit spoilage organisms and control the presence of foodborne pathogens. The proteome profiles were performed by two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF/TOF identification to determine the effects of HHP (50, 100, 150 and 200 MPa, each for 10 min) on Vibrio parahaemolyticus ATCC 17802 (~8 log cfu ml(-1) ) in order to understand how it responds to mechanical stress injury. Multiple comparisons of 2-DE revealed that the majority of changes in protein abundance occurred in a pressure-dependent fashion. A total of 18 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF analysis. Moreover, quantitative RT-PCR and immunoblotting were also demonstrated to substantiate the changes of transcriptional and translational levels of representative proteins. Our results suggested that V. parahaemolyticus may respond to HHP treatment through suppression of membrane stability and functionality (PfaC, Alr2, MltA, PLA2 and PatH), depression of biosynthesis and cellular processes (NadB, PyrB and ArgB), decreased levels of transcription (RpoD) and translation (RpsA, RplJ and PheS), and effective activation of protein folding and stress-related elements (GroES, DnaK and GroEL). This study may provide insight into the nature of the cellular targets of high pressure and in high-pressure resistance mechanisms in V. parahaemolyticus.
Authors:
Ling-Lin Fu; Rui Wang; Yanbo Wang; Junda Lin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-28
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  -     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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