| Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14. | |
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MedLine Citation:
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PMID: 20403971 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in a broad range of biological functions. Here we describe an extensive Cid14 protein-protein interaction network and its biochemical dissection. Cid14 most stably interacts with the zinc-knuckle protein Air1 to form the Cid14-Air1 complex (CAC). Providing a link to ribosomal RNA processing, Cid14 sediments with 60S ribosomal subunits and copurifies with 60S assembly factors. In contrast, no physical link to chromatin has been identified, although gene expression profiling revealed that efficient silencing of a few heterochromatic genes depends on Cid14 and/or Air1. |
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Authors:
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Claudia Keller; Katrina Woolcock; Daniel Hess; Marc Bühler |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-19 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 16 ISSN: 1469-9001 ISO Abbreviation: RNA Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-07-07 Revised Date: 2011-03-03 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 1124-9 Citation Subset: IM |
Affiliation:
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Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatin
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genetics,
metabolism Cycloheximide / metabolism Gene Expression Regulation, Fungal Kinetics Molecular Weight Polynucleotide Adenylyltransferase / chemistry*, metabolism Polyribosomes / metabolism Puromycin / metabolism RNA, Fungal / genetics, metabolism RNA, Ribosomal / genetics, metabolism Schizosaccharomyces / enzymology Schizosaccharomyces pombe Proteins / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/RNA, Fungal; 0/RNA, Ribosomal; 0/Schizosaccharomyces pombe Proteins; 53-79-2/Puromycin; 66-81-9/Cycloheximide; EC 2.7.7.19/Cid14 protein, S pombe; EC 2.7.7.19/Polynucleotide Adenylyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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