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Proteomic exploration of the impacts of pomegranate fruit juice on the global gene expression of prostate cancer cell.
MedLine Citation:
PMID:  22945439     Owner:  NLM     Status:  Publisher    
Prostate cancer has been known to be the second highest cause of death in cancer among men. Pomegranate is rich in polyphenols with the potent antioxidant activity and inhibits cell proliferation, invasion and promotes apoptosis in various cancer cells. This study demonstrated that pomegranate fruit juice could effectively hinder the proliferation of human prostate cancer DU145 cell. The results of apoptotic analyses implicated that fruit juice might trigger the apoptosis in DU145 cells via death receptor signaling and mitochondrial damage pathway. In this study we exploited two-dimensional gel electrophoresis-based proteomics to compare 9 pairs of the proteome maps collected from untreated- and treated-DU145 cells to identify the differentially expressed proteins. Comparative proteomics indicated that 11 proteins were de-regulated in affected DU145 cells with 3 up-regulated and 8 down-regulated proteins. These dys-regultaed proteins participated in cytoskeletal functions, anti-apoptosis, proteasome activity, NF-κB signaling, cancer cell proliferation, invasion and angiogenesis. Western immunoblotting were implemented to confirm the de-regulated proteins and the down-stream signaling proteins. The analytical results of this study help to provide insight into the molecular mechanism of inducing prostate cancer cell apoptosis by pomegranate fruit juice and to develop novel mechanism-based chemopreventive strategy for prostate cancer.
Song-Tay Lee; Yi-Ling Wu; Lan-Hsiang Chien; Szu-Ting Chen; Yu-Kai Tzeng; Ting-Feng Wu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-4
Journal Detail:
Title:  Proteomics     Volume:  -     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
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