Document Detail


Proteomic discovery of protease substrates.
MedLine Citation:
PMID:  17194619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Elucidation of in vivo substrate degradomes of a protease is a daunting endeavor because of the large number of proteins in a proteome and often minute and transient amounts of key substrates. Proteomic substrate screens for proteases are currently experiencing impressive progress. Mass spectrometry-based global proteome analysis, interfaced with liquid-chromatography and together with stable isotope labeling strategies, has provided increased coverage and sensitivity for quantitative proteomics. ICAT and iTRAQ labeling have been used to identify a plethora of new matrix metalloproteinase substrates. Emerging techniques focus on the quantitative analysis of proteolytically generated neo amino-termini, which we call terminopes, on a system-wide basis. In vivo SILAC pulse-chase experiments have also enabled the study of individual protein turnover and global proteome dynamics in cells and whole organisms. Together with activity-based probes for the profiling of functional proteases, there is now in place an array of complementary technologies to dissect the 'protease web' and its distortion in pathology.
Authors:
Oliver Schilling; Christopher M Overall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2006-12-27
Journal Detail:
Title:  Current opinion in chemical biology     Volume:  11     ISSN:  1367-5931     ISO Abbreviation:  Curr Opin Chem Biol     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-12     Completed Date:  2007-05-01     Revised Date:  2009-08-25    
Medline Journal Info:
Nlm Unique ID:  9811312     Medline TA:  Curr Opin Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  36-45     Citation Subset:  IM    
Affiliation:
The UBC Centre for Blood Research, Departments of Oral Biological & Medical Sciences, and Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid / methods
Indicators and Reagents
Isotope Labeling*
Models, Biological
Peptide Hydrolases / chemistry,  genetics,  metabolism*
Proteomics / methods*,  trends
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Substrate Specificity
Chemical
Reg. No./Substance:
0/Indicators and Reagents; EC 3.4.-/Peptide Hydrolases

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