| Proteomic differentiation pattern in the U937 cell line. | |
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MedLine Citation:
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PMID: 20801507 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The U937 cell line, originally established from a histiocytic lymphoma, has been widely used as a powerful in vitro model for haematological studies. These cells retain the immature cell phenotype and can be induced to differentiate by several factors, among which 12-O-tetradecanoyl-13-phorbol acetate (TPA). Fully differentiated cells acquire the adherent phenotype and exhibit various properties typical of macrophages. However, in spite of a great deal of research devoted to the U937 cellular model, the molecular basis of biological processes involved in the monocyte/macrophage differentiation remains unclear. The present study has been undertaken to contribute to this knowledge, in order to identify proteomic-based differentiation pattern for the U937 cells exposed to TPA. Present results have highlighted that the U937 cell differentiation is correlated with a significant proteomic modulation, corresponding to about 30% of the identified proteins, including both over- and down-regulated proteins. Negative modulation regarded proteins involved in the regulation of cell proliferation and in metabolic processes. Proteins appearing incremented in macrophagic phenotype include calcium- and phospholipid-binding proteins and several proteins related to the phagocytic activity. Conclusively, we suggest that this new set of differentially expressed proteins may represent meaningful myelo-monocytic differentiation markers to be applied to the study of several haematological diseases. |
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Authors:
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Luigi Minafra; Gianluca Di Cara; Nadia Ninfa Albanese; Patrizia Cancemi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Leukemia research Volume: 35 ISSN: 1873-5835 ISO Abbreviation: Leuk. Res. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7706787 Medline TA: Leuk Res Country: England |
Other Details:
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Languages: eng Pagination: 226-36 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Dipartimento di Oncologia Sperimentale ed Applicazioni Cliniche, Università di Palermo, Palermo, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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