Document Detail


Proteomic characterization of <italic>Aspergillus fumigatus</italic> treated with an antifungal coumarin for identification of novel target molecules of key pathways.
MedLine Citation:
PMID:  22533410     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A synthetic coumarin, N,N,N-triethyl-11-(4-methyl-2-oxo-2H-chromen-7-yloxy)-11-oxoundecan-1-aminium bromide (SCD-1) having potent activity against pathogenic <italic>Aspergilli</italic> (MIC<sub>90</sub> 15.62 µg/ml) was investigated to identify its molecular targets in the pathogen. The proteome of <italic>Aspergillus fumigatus</italic> was developed after treatment with sub-lethal doses of compound and analyzed. The results demonstrated 143 differentially expressed proteins on treatment with SCD-1. The expression of 4 proteins namely cell division control protein, ubiquitin-like activating enzyme, vacuolar ATP synthase catalytic subunit A and UTP-glucose-1-phosphate uridylyltransferase of <italic>A. fumigatus</italic>, was completely inhibited, whereas there were 13 newly expressed and 96 over expressed proteins, mainly belonging to stress pathway. The treatment of <italic>A. fumigatus</italic> with SCD-1 also led to attenuation of proteins involved in cell replication and other important biosynthetic processes including riboflavin biosynthesis which has been pathogen-specific. In addition to key enzymatic players and antioxidants, 9 hypothetical proteins were also identified, 7 of which have been novel being described for the first time. As no cellular functions have yet been described for these hypothetical proteins, their alteration in response to SCD-1 provides significant information about their putative roles in pathogen defense.
Authors:
Seema Singh; Shilpi Gupta; Bharat Singh; Sunil K Sharma; Vijay Kumar Gupta; Gainda Lal Sharma
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-25
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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