| Proteomic characterization of <italic>Aspergillus fumigatus</italic> treated with an antifungal coumarin for identification of novel target molecules of key pathways. | |
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MedLine Citation:
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PMID: 22533410 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A synthetic coumarin, N,N,N-triethyl-11-(4-methyl-2-oxo-2H-chromen-7-yloxy)-11-oxoundecan-1-aminium bromide (SCD-1) having potent activity against pathogenic <italic>Aspergilli</italic> (MIC<sub>90</sub> 15.62 µg/ml) was investigated to identify its molecular targets in the pathogen. The proteome of <italic>Aspergillus fumigatus</italic> was developed after treatment with sub-lethal doses of compound and analyzed. The results demonstrated 143 differentially expressed proteins on treatment with SCD-1. The expression of 4 proteins namely cell division control protein, ubiquitin-like activating enzyme, vacuolar ATP synthase catalytic subunit A and UTP-glucose-1-phosphate uridylyltransferase of <italic>A. fumigatus</italic>, was completely inhibited, whereas there were 13 newly expressed and 96 over expressed proteins, mainly belonging to stress pathway. The treatment of <italic>A. fumigatus</italic> with SCD-1 also led to attenuation of proteins involved in cell replication and other important biosynthetic processes including riboflavin biosynthesis which has been pathogen-specific. In addition to key enzymatic players and antioxidants, 9 hypothetical proteins were also identified, 7 of which have been novel being described for the first time. As no cellular functions have yet been described for these hypothetical proteins, their alteration in response to SCD-1 provides significant information about their putative roles in pathogen defense. |
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Authors:
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Seema Singh; Shilpi Gupta; Bharat Singh; Sunil K Sharma; Vijay Kumar Gupta; Gainda Lal Sharma |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-25 |
Journal Detail:
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Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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