| Proteomic characterization of Jurkat T leukemic cells after dopamine stimulation: a model of circulating dopamine-sensitive cells. | |
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MedLine Citation:
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PMID: 21300132 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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T-cells are circulating dopamine-sensitivecells and may mirror, at the peripheral level, biochemical modifications occurring in dopaminergic neurons. The human CD4+ T leukemic Jurkat cell line has been thoroughly used and characterized as a suitable cell model to investigate T-cell signaling and apoptosis. Here, we describe their characterization as a model of circulating dopamine-sensitivecells and their response to a dopamine challenge by analyzing changes in the cell proteome. Jurkat cells express both D1- and D2-like dopamine receptors and both membrane and vesicular transporters, while they lack D4 receptor and tyrosine hydroxylase expression. A saturating, non-toxic, non-oxidant 50 μM dopamine challenge induces the upregulation of an interacting chaperone network known to protect specifically dopaminergic neurons, thus validating T-cells as a biomarker discovery source in Parkinson's disease. Remodeling of the distribution pattern of β-actin and 14-3-3 isoforms is consistently observed upon dopamine treatment. As a whole, dopamine-specific alterations here observed might represent a biosensor for dopamine response at the peripheral level. |
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Authors:
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Tiziana Alberio; Claudia Anchieri; Luca Piacentini; Giovanna Gentile; Maurizio Simmaco; Mara Biasin; Mauro Fasano |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-4 |
Journal Detail:
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Title: Biochimie Volume: - ISSN: 1638-6183 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria via Alberto da Giussano 12, 21052 Busto Arsizio, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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