| Proteomic analysis shows the upregulation of erythrocyte dematin in zinc-restricted human subjects. | |
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MedLine Citation:
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PMID: 22456662 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Although the importance of adequate zinc intake has been known for decades, the estimated global prevalence of zinc deficiency remains high. This substantiates the need for a specific and sensitive status assessment tool. OBJECTIVE: The objective was to evaluate erythrocyte zinc transporters as candidate molecules with the potential of being a biomarker of dietary zinc status in humans. DESIGN: A 24-d observational study with acclimation (7 d, 10.4 mg Zn/d), zinc-depletion (10 d, 0.3 mg Zn/d), and zinc-repletion (7 d, 29.5 mg Zn/d) phases was conducted in healthy men (n = 9). Proteomic approaches including Western blot analyses and tandem mass spectrometry were implemented to identify the zinc responsiveness of selected red blood cell membrane proteins. RESULTS: Zinc transporter 1 (ZnT1) and Zrt/Irt-like proteins ZIP8 and ZIP10 were detected in human erythrocyte membranes. No effects of short-term dietary zinc depletion were observed on the amounts of these proteins. However, changes in a cytoskeletal protein, dematin, by zinc depletion were identified through the nonspecific signals produced by an anti-ZIP8 antibody. This response was further validated by a dematin-specific antibody and with erythrocytes collected from mice fed a zinc-deficient diet. CONCLUSIONS: The presence of ZnT1, ZIP8, and ZIP10 in human red blood cells implicates their role in the regulation of cellular zinc metabolism in the human erythroid system. The zinc responsiveness of membrane dematin suggests its capability to serve as a biomarker for dietary zinc depletion and its involvement in impaired erythroid membrane fragility by zinc restriction. This trial was registered at clinicaltrials.gov as NCT01221129. |
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Authors:
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Moon-Suhn Ryu; Gregory J Guthrie; Alyssa B Maki; Tolunay B Aydemir; Robert J Cousins |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-03-28 |
Journal Detail:
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Title: The American journal of clinical nutrition Volume: 95 ISSN: 1938-3207 ISO Abbreviation: Am. J. Clin. Nutr. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-04-23 Completed Date: 2012-06-14 Revised Date: 2013-06-14 |
Medline Journal Info:
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Nlm Unique ID: 0376027 Medline TA: Am J Clin Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1096-102 Citation Subset: AIM; IM |
Affiliation:
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Food Science and Human Nutrition Department, Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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ClinicalTrials.gov/NCT01221129 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amino Acid Sequence Animals Biological Markers / blood Blotting, Western Carrier Proteins / metabolism Cation Transport Proteins / metabolism Erythrocytes / metabolism* Humans Male Mice Mice, Inbred C57BL Microfilament Proteins / metabolism* Molecular Sequence Data Nutritional Status Proteome / analysis* Tandem Mass Spectrometry Up-Regulation Young Adult Zinc / administration & dosage*, deficiency* |
| Grant Support | |
ID/Acronym/Agency:
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DK31127/DK/NIDDK NIH HHS; DK94244/DK/NIDDK NIH HHS; R01 DK094244/DK/NIDDK NIH HHS; RR029890/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Carrier Proteins; 0/Cation Transport Proteins; 0/EPB49 protein, human; 0/Microfilament Proteins; 0/Proteome; 0/SLC30A1 protein, human; 0/SLC39A10 protein, human; 0/Zip8 protein, human; 0/zinc-binding protein; 7440-66-6/Zinc |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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