Document Detail

Proteomic analysis of seminal plasma from asthenozoospermia patients reveals proteins that affect oxidative stress responses and semen quality.
MedLine Citation:
PMID:  19448646     Owner:  NLM     Status:  MEDLINE    
Asthenozoospermia (AS) is a common cause of human male infertility. In one study, more than 80% of the samples from infertile men had reduced sperm motility. Seminal plasma is a mixture of secretions from the testis, epididymis and several male accessory glands, including the prostate, seminal vesicles and Cowper's gland. Studies have shown that seminal plasma contains proteins that are important for sperm motility. To further explore the pathophysiological character of AS, we separated the seminal plasma proteins from AS patients and healthy donors using sodium dodecyl sulfate polyacrylamide gel electrophoresis and in-gel digestion, and then subjected the proteins to liquid chromatography-mass spectrometry (LC-MS/MS) analysis. A total of 741 proteins were identified in the seminal plasma, with a false discovery rate of 3.3%. Using spectral counting, we found that 45 proteins were threefold upregulated and 56 proteins were threefold downregulated in the AS group when compared with the control. Most of these proteins originated from the epididymis and prostate. This study identified a rich source of biomarker candidates for male infertility and indicates that functional abnormalities of the epididymis and prostate can contribute to AS. We identified DJ-1-a protein that has been shown elsewhere to be involved in the control of oxidative stress (OS)-as a downregulated protein in AS seminal plasma. The levels of DJ-1 in AS seminal plasma were about half of those in the control samples. In addition, the levels of reactive oxygen species were 3.3-fold higher in the AS samples than in the controls. Taken together, these data suggest that downregulation of DJ-1 is involved in OS in semen, and therefore affects the quality of the semen.
Jun Wang; Jian Wang; Hua-Rong Zhang; Hui-Juan Shi; Duan Ma; Hong-Xin Zhao; Biaoyang Lin; Run-Sheng Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-18
Journal Detail:
Title:  Asian journal of andrology     Volume:  11     ISSN:  1745-7262     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-03     Completed Date:  2009-12-07     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  484-91     Citation Subset:  IM    
Systems Biology Division, Zhejiang-California Nanosystems Institute (ZCNI) Zhejiang University, Hangzhou 310029, China.
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MeSH Terms
Asthenozoospermia / metabolism*
Blotting, Western
Chromatography, Liquid
Oxidative Stress*
Proteins / metabolism*
Reactive Oxygen Species / metabolism
Semen / metabolism*
Tandem Mass Spectrometry
Reg. No./Substance:
0/Proteins; 0/Reactive Oxygen Species

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