Document Detail


Proteomic analysis reveals that apolipoprotein A1 levels are decreased in patients with Budd-Chiari syndrome.
MedLine Citation:
PMID:  21145806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND & AIMS: Budd-Chiari syndrome (BCS) is a rare vascular liver disorder caused by thrombosis of the hepatic veins. In some patients, no known thrombophilic factor can be identified. This study aimed to identify novel factors that might play a role in thrombosis in BCS-patients by using a proteomic approach.
METHODS: The abundance of plasma clot-bound proteins was compared between nine BCS-patients and nine controls by using two-dimensional difference gel electrophoresis. The protein with the most significant decrease in patients was identified by mass spectrometry. Plasma levels of this protein were measured and the results were validated in a large cohort of BCS-patients.
RESULTS: A total of 26 protein spots significantly differed (p<0.001). The spot that decreased with the highest statistical significance in patients was identified by mass spectrometry as apolipoprotein A1 (apo A1). The mean level of apo A1 in the plasma of these BCS-patients (0.74 g/L) was also significantly lower than in controls (1.45 g/L, p=0.002). This finding was validated in a large cohort of 101 BCS-patients and 101 controls (0.97 g/L vs. 1.32 g/L, p<0.0001). There was no major correlation between plasma levels of apo A1 and various liver function tests.
CONCLUSIONS: BCS-patients show decreased clot-bound protein abundance and plasma levels of apo A1. Decreased levels of apo A1 may play a role in the etiology of thrombosis in BCS-patients and possibly in other patients with venous thrombosis.
Authors:
Simone Talens; Jildou Hoekstra; Steffen P G Dirkx; Sarwa Darwish Murad; Jonel Trebicka; Elwyn Elias; Massimo Primignani; Juan-Carlos García-Pagán; Dominique C Valla; Harry L A Janssen; Frank W G Leebeek; Dingeman C Rijken;
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-14
Journal Detail:
Title:  Journal of hepatology     Volume:  54     ISSN:  1600-0641     ISO Abbreviation:  J. Hepatol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-18     Completed Date:  2011-08-17     Revised Date:  2013-01-11    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  908-14     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Apolipoprotein A-I / blood*
Budd-Chiari Syndrome / blood*,  complications*
Case-Control Studies
Cholesterol, HDL / blood
Cohort Studies
Electrophoresis, Gel, Two-Dimensional
Hepatic Veins
Humans
Proteomics
Thrombosis / blood*,  etiology*
Chemical
Reg. No./Substance:
0/APOA1 protein, human; 0/Apolipoprotein A-I; 0/Cholesterol, HDL
Investigator
Investigator/Affiliation:
D C Valla / ; H L A Janssen / ; J C Garcia-Pagan / ; P Langlet / ; L Lasser / ; D C Valla / ; A Plessier / ; B Condat / ; M Bahr / ; J Heller / ; J Trebicka / ; M Rössle / ; M Primignani / ; F Fabris / ; H L A Janssen / ; S Darwish Murad / ; J Hoekstra / ; F W G Leebeek / ; F R Rosendaal / ; H Miranda / ; J C Garcia-Pagan / ; M Hernandez-Guerra / ; S Raffa / ; R Lozano / ; A Hadengue / ; I Morard / ; G Mentha / ; E Elias / ; C E Eapen /

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