Document Detail


Proteomic analysis of rat skeletal muscle submitted to one bout of incremental exercise.
MedLine Citation:
PMID:  20973830     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Exercise can alter gene transcriptional and protein translational rates leading to changes in protein abundance toward adaptation to exercise. We investigated the alterations in protein abundance in skeletal muscle after one bout of an exhaustive exercise through proteomic analysis. Gastrocnemius muscles were sampled from non-exercised control rats and from rats exercised on a treadmill with incremental increases in speed until exhaustion (approximately 30 min). Rats were sacrificed 3 and 24 h after exercise cessation. Two-dimensional gel electrophoresis was performed and spots with a significant alteration in relative volume were identified by mass spectrometry. Six spots presented statistically significant altered abundances after exercise. The spots identified as the metabolic related proteins triosephosphate isomerase 1, glyceraldehyde-3-phosphate dehydrogenase, the β subunit of pyruvate dehydrogenase E(1) and carnitine palmitoyltransferase 2 were all more abundant after exercise. One spot identified as heat shock cognate 70 was also more abundant after exercise. One spot demonstrated a decreased abundance after exercise and was identified as α-actin. These results suggest that a single session of exhaustive incremental exercise in untrained muscle can alter thin filaments synthesis/degradation rate and enhance cytosolic and mitochondrial proteins synthesis. The identified proteins may be important to a general preconditioning of skeletal muscle for subsequent exercise sessions.
Authors:
P G Gandra; R H Valente; J Perales; A G Pacheco; D V Macedo
Publication Detail:
Type:  Journal Article     Date:  2010-10-25
Journal Detail:
Title:  Scandinavian journal of medicine & science in sports     Volume:  22     ISSN:  1600-0838     ISO Abbreviation:  Scand J Med Sci Sports     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111504     Medline TA:  Scand J Med Sci Sports     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  207-16     Citation Subset:  IM    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Affiliation:
Laboratório de Bioquímica do Exercício (LABEX), Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, BrazilLaboratório de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365, Rio de Janeiro, BrazilPrograma de Computação Científica, Fiocruz, Av. Brasil 4365, Rio de Janeiro, Brazil.
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