Document Detail

Proteomic analysis of rabbit tear fluid: Defensin levels after an experimental corneal wound are correlated to wound closure.
MedLine Citation:
PMID:  17676663     Owner:  NLM     Status:  MEDLINE    
The cornea is the major refracting optical element of the eye and therefore critical for forming a retinal image. The exposed surface of the eye is protected from pathogens by the innate immune system whose components include defensins, naturally occurring peptides with antimicrobial properties, and the physical barrier formed by the outer epithelial layer of the cornea. The proteomic approach has revealed that tear levels of defensins are correlated with the course of healing of an experimental corneal wound. Tears were collected from New Zealand White rabbits prior to (day 0) and daily for 5 days (days 1-5) following a standard unilateral 6 mm diameter corneal epithelial abrasion. Tear protein profiles obtained from wounded and contra-lateral control eyes were compared using SELDI ProteinChip technology. Peptides and proteins of interest were purified by RP-HPLC and characterized by nanoESI-MS/MS. Mass spectra of tears on post-wound day 1, revealed 13 peaks whose level decreased and five that increased. During wound healing the tear protein profile correlated with wound closure. An important finding was that the levels of rabbit defensins (NP-1 and NP-2), which were elevated after wounding returned to normal levels by the time the corneal abrasion healed. Relative quantification of NP-2 in tear fluid prior to (day 0) and after corneal wounding (days 1- 3) was determined using iTRAQ technology. A corneal wound eliminates the barrier function of innate immunity and puts the cornea at risk from microbial attack until the epithelial cells restore the surface barrier. The increased availability of defensins in the tears during healing suggests that these peptides could protect the cornea from microbial attack during a period of increased vulnerability.
Lei Zhou; Roger W Beuerman; Liqun Huang; Amutha Barathi; Yong Hwee Foo; Sam F Y Li; Fook Tim Chew; Donald Tan
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Proteomics     Volume:  7     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-12     Completed Date:  2007-11-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3194-206     Citation Subset:  IM    
Singapore Eye Research Institute, Singapore.
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MeSH Terms
Amino Acid Sequence
Anti-Infective Agents / analysis*,  chemistry
Chromatography, High Pressure Liquid
Cornea / injuries,  metabolism
Defensins / analysis*,  chemistry
Disulfides / chemistry
Molecular Sequence Data
Molecular Weight
Nanotechnology / methods
Proteomics / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Tears / chemistry*
Time Factors
Trypsin / pharmacology
Wound Healing / physiology*
Reg. No./Substance:
0/Anti-Infective Agents; 0/Defensins; 0/Disulfides; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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