Document Detail


Proteomic analysis of preharvest sprouting in rye using two-dimensional electrophoresis and mass spectrometry.
MedLine Citation:
PMID:  23024596     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Qualitative and quantitative differences were found between two-dimensional electrophoretic spectra of 546 proteins from two bulked samples of mature rye grain representing: (1) 20 recombinant inbred lines extremely resistant to preharvest sprouting and (2) 20 recombinant inbred lines extremely susceptible to preharvest sprouting. Mass spectrometry of resolved proteins showed that four spots specific for PHS susceptibility represented high molecular weight glutenin subunit, glutathione transferase, 16.9 kDa heat-shock protein, and monomeric alpha-amylase inhibitor. Two spots specific for PHS resistance contained cytosolic malate dehydrogenase and functionally unrecognized protein with sequence homology to rubber elongation factor protein. Majority of 14 proteins with at least two-fold higher accumulation level in preharvest sprouting susceptible lines relative to that found in sprouting resistant lines, showed sequence homology to proteins involved in defense mechanisms against biotic and abiotic stresses including oxidative stress, and those taking part in energy supply. Two spots were identified as regulatory proteins from the 14-3-3 family with one molecular form prevailing in sprouting susceptible and another form highly accumulated in sprouting resistant lines. Further study establishing map positions of the revealed structural genes in respect to quantitative trait loci for preharvest sprouting in rye should answer the question on their possible status as candidate genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-012-9721-z) contains supplementary material, which is available to authorized users.
Authors:
Piotr Masojć; Arkadiusz Kosmala
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-14
Journal Detail:
Title:  Molecular breeding : new strategies in plant improvement     Volume:  30     ISSN:  1380-3743     ISO Abbreviation:  Mol. Breed.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506703     Medline TA:  Mol Breed     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1355-1361     Citation Subset:  -    
Affiliation:
West Pomeranian University of Technology, Słowackiego 17, 71-434 Szczecin, Poland.
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