Document Detail

Proteomic analysis of pancreatic endocrine tumor cell lines treated with the histone deacetylase inhibitor trichostatin A.
MedLine Citation:
PMID:  17443844     Owner:  NLM     Status:  MEDLINE    
Effects of the histone-deacetylases inhibitor trichostatin A (TSA) on the growth of three different human pancreatic endocrine carcinoma cell lines (CM, BON, and QGP-1) have been assessed via dosage-dependent growth inhibition curves. TSA determined strong inhibition of cell growth with similar IC(50) values for the different cell lines: 80.5 nM (CM), 61.6 nM (BON), and 86 nM (QGP-1), by arresting the cell cycle in G2/M phase and inducing apoptosis. 2DE and nano-RP-HPLC-ESI-MS/MS analysis revealed 34, 33, and 38 unique proteins differentially expressed after TSA treatment in the CM, BON, and QGP-1 cell lines, respectively. The most important groups of modulated proteins belong to cell proliferation, cell cycle, and apoptosis classes (such as peroxiredoxins 1 and 2, the diablo protein, and HSP27). Other proteins pertain to processes such as regulation of gene expression (nucleophosmin, oncoprotein dek), signal transduction (calcium-calmodulin), chromatin, and cytoskeleton organization (calgizzarin, dynein, and lamin), RNA splicing (nucleolin, HNRPC), and protein folding (HSP70). The present data are in agreement with previous proteomic analyses performed on pancreatic ductal carcinoma cell lines (Cecconi, D. et al.., Electrophoresis 2003; Cecconi, D. et al., J. Proteome Res. 2005) and place histone-deacetylases inhibitors among the potentially most powerful drugs for the treatment of pancreatic tumors.
Daniela Cecconi; Massimo Donadelli; Sara Rinalducci; Lello Zolla; Maria Teresa Scupoli; Aldo Scarpa; Marta Palmieri; Pier Giorgio Righetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  7     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-07-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1644-53     Citation Subset:  IM    
Department of Science and Technologies, Section of Biochemical Methodologies, University of Verona, Verona, Italy.
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MeSH Terms
Apoptosis / physiology
Cell Line, Tumor / drug effects*
Cell Proliferation
Cluster Analysis
Electrophoresis, Gel, Two-Dimensional
Endocrine Gland Neoplasms / chemistry*
Enzyme Inhibitors / pharmacology*
Histone Deacetylase Inhibitors
Hydroxamic Acids / pharmacology*
Mass Spectrometry
Pancreatic Neoplasms / chemistry*
Proteome / analysis*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/Proteome; 58880-19-6/trichostatin A

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