Document Detail


Proteomic analysis of left ventricular tissues following intermittent myocardial ischemia during coronary collateralization in rabbits.
MedLine Citation:
PMID:  18207584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Repeated transient myocardial ischemia may offer favorable effects to coronary perfusion via collateral circulation, although the underlying molecular mechanisms still remain unclear. This study was designed to evaluate the proteomic changes during this process. METHODS: Rabbits were randomly divided into sham-operated and ischemic groups (5 each) and were subjected to intermittent myocardial ischemia by inflation or deflation of pneumatic occluders for 4 weeks to establish a controlled myocardial ischemic model. Isolated hearts were subjected to histological observation, microspheric detection, capillary counting and proteomic analysis. RESULTS: Elevation of ST segment or back to normal in Lead-II electrocardiogram could be induced by occluders without overt histological and cardiac troponin I alterations. Regional coronary collateral blood flow exhibited a remarkable increase following intermittent inflation of occluders in the ischemic group (P<0.01). Simultaneously, capillary numbers per unit area were significantly different between groups (P<0.01). Twenty-three differentially expressed protein spots were separated by two-dimensional gel electrophoresis and 13 out of them were identified by MALDI-TOF-MS. CONCLUSION: The present study indicates that the differentially expressed proteins involved in proliferation, growth and energy metabolism following intermittent myocardial ischemia without ischemia-reperfusion injury are likely associated with the development of collateralization beneficial to coronary circulation.
Authors:
Jinyang Gu; Yuanhui Wang; Jianan Li; Jun Wang; Tingjian Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-22
Journal Detail:
Title:  International journal of cardiology     Volume:  131     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  326-35     Citation Subset:  IM    
Affiliation:
Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillaries / pathology
Collateral Circulation*
Coronary Circulation*
Electrocardiography
Electrophoresis, Gel, Two-Dimensional
Female
Heart Ventricles
Intercellular Signaling Peptides and Proteins / metabolism
Male
Muscle Proteins / metabolism
Myocardial Ischemia / metabolism,  pathology,  physiopathology*
Myocardium / metabolism*,  pathology
Proteomics
Rabbits
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Troponin I / metabolism
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Muscle Proteins; 0/Troponin I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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