| Proteomic analysis of latex from the rubber-producing plant Taraxacum brevicorniculatum. | |
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MedLine Citation:
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PMID: 22539439 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Many plants produce latex, a specialized, metabolically active cytoplasm. This is generally regarded as a defensive trait but latex may also possess additional functions. We investigated the role of latex in the dandelion species Taraxacum brevicorniculatum that contains considerable amounts of high-quality natural rubber by carrying out a comprehensive analysis of the latex proteome. We developed reliable protocols for the preparation of protein samples for one-dimensional gel electrophoresis, two-dimensional gel electrophoresis, and subsequent mass spectrometry analysis, which led to 278 unique identifications. A gene ontology classification system based on comparisons with known Arabidopsis thaliana root proteins showed that dandelion proteins involved in lipid metabolism and transport were enriched in the latex proteome, whereas those involved in stress responses were not. We also found that proteins involved in rubber biosynthesis were distributed among different fractions of the latex proteome. |
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Authors:
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Daniela Wahler; Thomas Colby; Natalie A Kowalski; Anne Harzen; Sandra Y Wotzka; Andrea Hillebrand; Rainer Fischer; Johannes Helsper; Jürgen Schmidt; Christian Schulze Gronover; Dirk Prüfer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proteomics Volume: 12 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-04-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 901-5 Citation Subset: IM |
Copyright Information:
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© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Molecular Biotechnology, RWTH Aachen, Aachen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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