Document Detail


Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry.
MedLine Citation:
PMID:  22096114     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lung cancer is often asymptomatic or causes only nonspecific symptoms in its early stages. Early detection represents one of the most promising approaches to reduce the growing lung cancer burden. Human saliva is an attractive diagnostic fluid because its collection is less invasive than that of tissue or blood. Profiling of proteins in saliva over the course of disease progression could reveal potential biomarkers indicative of oral or systematic diseases, which may be used extensively in future medical diagnostics. There were 72 subjects enrolled in this study for saliva sample collection according to the approved protocol. Two-dimensional difference gel electrophoresis combined with MS was the platform for salivary proteome separation, quantification, and identification from two pooled samples. Candidate proteomic biomarkers were verified and prevalidated by using immunoassay methods. There were 16 candidate protein biomarkers discovered by two-dimensional difference gel electrophoresis and MS. Three proteins were further verified in the discovery sample set, prevalidation sample set, and lung cancer cell lines. The discriminatory power of these candidate biomarkers in lung cancer patients and healthy control subjects can reach 88.5% sensitivity and 92.3% specificity with AUC = 0.90. This preliminary data report demonstrates that proteomic biomarkers are present in human saliva when people develop lung cancer. The discriminatory power of these candidate biomarkers indicate that a simple saliva test might be established for lung cancer clinical screening and detection.
Authors:
Hua Xiao; Lei Zhang; Hui Zhou; Jay M Lee; Edward B Garon; David T W Wong
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-17
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  11     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-07     Completed Date:  2012-06-01     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M111.012112     Citation Subset:  IM    
Affiliation:
Dental Research Institute, University of California, Los Angeles, Los Angeles, California 90095, USA. xiaohuacn@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Blotting, Western
Carcinoma, Non-Small-Cell Lung / metabolism*
Case-Control Studies
Cells, Cultured
Early Detection of Cancer
Enzyme-Linked Immunosorbent Assay
Female
Humans
Lung / metabolism*
Lung Neoplasms / metabolism*
Male
Middle Aged
Proteome / analysis*
Saliva / chemistry,  metabolism*
Small Cell Lung Carcinoma / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tumor Markers, Biological / metabolism*
Two-Dimensional Difference Gel Electrophoresis
Grant Support
ID/Acronym/Agency:
R21-CA0126733/CA/NCI NIH HHS; U01-DE16275/DE/NIDCR NIH HHS; UL1 TR000124/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Proteome; 0/Tumor Markers, Biological
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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