Document Detail

Proteomic analysis of chondrocytes exposed to pressure.
MedLine Citation:
PMID:  21077246     Owner:  NLM     Status:  MEDLINE    
Chondrocytes are the only cell type present in mature articular cartilage (2-5% of total tissue). The biological activities of the chondrocyte population are regulated by genetic, biologic and biochemical factors, as well as environmental factors (stress, flow and electric field). Although compressive forces within joint articular cartilage are required for maintenance of the normal composition of articular cartilage, there is a lack of knowledge about the number of pressure-related proteins expressed in articular cartilage. Two-dimensional gel electrophoresis (2-DE) and high-performance liquid chromatography-electrospray/tandem mass spectrometry (HPLC/ESI-MS/MS) were used to identify the levels of pressure-related proteins expressed by chondrocytes grown in the presence or absence of hydrostatic pressure. A total of 266 spots were excised from the gels and analyzed by HPLC/ESI-MS/MS. Functional classification of up-regulated proteins indicated that energy and protein fate were the main biological processes occurring in pressurized chondrocytes. Furthermore, membrane-bound transferrin-like protein p97, a marker of chondrocyte differentiation, was only expressed in chondrocytes under hydrostatic pressure. These data suggest that hydrostatic pressure can induce cell differentiation by increasing the expression level of energy metabolism- and protein fate-related proteins, indicating that hydrostatic pressure may be needed for normal biosynthesis and differentiation of articular chondrocytes.
Yu-Min Juang; Chun-Yi Lee; Wei-Yi Hsu; Chiung-Tsung Lin; Chien-Chen Lai; Fuu-Jen Tsai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  24     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-15     Completed Date:  2011-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  1273-82     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.
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MeSH Terms
Cartilage, Articular / chemistry,  cytology,  metabolism
Cell Differentiation
Cells, Cultured
Chondrocytes / chemistry*,  cytology,  metabolism*
Chromatography, High Pressure Liquid
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation
Hydrostatic Pressure
Molecular Sequence Data
Proteins / chemistry,  genetics,  metabolism
Proteomics* / instrumentation
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:

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