Document Detail

Proteomic analysis of the chicken egg vitelline membrane.
MedLine Citation:
PMID:  18452232     Owner:  NLM     Status:  MEDLINE    
The avian vitelline membrane (VM) is a multilayered proteinaceous structure separating egg white from yolk. The innermost layer of the VM, deposited onto the oocyte plasma membrane in the ovary, corresponds to the mammalian zona pellucida (ZP). The outer layer is produced in the infundibulum, the first section of the oviduct. Using high-throughput, high-end LC-MS(n) 137 proteins were identified, only 13 of which were known previously to be components of the VM. Depending on the washing protocol, two largely overlapping, but not identical, sets of identified proteins were produced from water-washed and salt-washed VMs. Most of the components of the VM were known previously from other egg compartments, such as, for instance, the egg white proteins lysozyme C, ovalbumin, ovotransferrin, and ovomucin. Specific components of the VM not identified previously in other egg compartments included eight ZP proteins, oviductin protease, and two ATPases. The vitelline outer membrane protein (VMO) VMO II was identified as beta-defensin-11. The list of VM proteins presented in this report is by far the most comprehensive dataset available at present and complements proteomic analyses of chicken egg compartments published previously.
Karlheinz Mann
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proteomics     Volume:  8     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-10     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2322-32     Citation Subset:  IM    
Max-Planck-Institut für Biochemie, Abteilung Proteomics und Signaltransduktion, Martinsried, Germany.
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MeSH Terms
Avian Proteins / chemistry*
Cell Membrane / metabolism
Egg Proteins / chemistry*
Egg Shell
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation*
Proteomics / methods*
Salts / pharmacology
Vitelline Membrane / metabolism*
Zona Pellucida / metabolism
Reg. No./Substance:
0/Avian Proteins; 0/Egg Proteins; 0/Proteome; 0/Salts; 0/vitelline membrane proteins

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