Document Detail


Proteomic analysis of asexual development of Phytophthora palmivora.
MedLine Citation:
PMID:  12825510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two-dimensional gel electrophoresis was used to analyse stage-specific proteins from Phytophthora palmivora, a pathogen of cocoa and other economically important tropical crops. Approximately 1% of proteins appeared to be specific for each of the mycelial, sporangial, zoospore, cyst and germinated cyst stages of the life-cycle. Three proteins excised from protein gels of P. palmivora were identified as isoforms of actin by database searches to public libraries of Phytophthora infestans. The protein profiles of parallel samples of P. palmivora and P. infestans demonstrated that 30% of proteins precisely co-migrated suggesting that proteomics may be used to examine changes in the specific stages in the life cycles of Phytophthora spp.
Authors:
Samantha J Shepherd; Pieter van West; Neil A R Gow
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mycological research     Volume:  107     ISSN:  0953-7562     ISO Abbreviation:  Mycol. Res.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-06-26     Completed Date:  2003-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8913481     Medline TA:  Mycol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Actins / isolation & purification
Algal Proteins / analysis*
Cacao / microbiology
Databases, Protein
Electrophoresis, Gel, Two-Dimensional
Phytophthora / chemistry*,  growth & development
Protein Isoforms / isolation & purification
Proteome / analysis*
Chemical
Reg. No./Substance:
0/Actins; 0/Algal Proteins; 0/Protein Isoforms; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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