Document Detail


Proteomic analysis of Tityus discrepans scorpion venom and amino acid sequence of novel toxins.
MedLine Citation:
PMID:  16705749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Venezuelan scorpion Tityus discrepans is known to cause human fatalities. We describe the first complete proteomic analysis of its venom. By HPLC 58 different fractions were obtained and 205 different components were identified by MS analysis. Components having molecular masses from 272 to 57 908 amu were found. Forty homogeneous components had their N-terminal amino acid sequence determined by Edman degradation, from which two new peptides named TdK2 and TdK3 (meaning T. discrepans (Td) K(+) channel toxins 2 and 3) were fully characterized. The first contains 34 amino acid residues with a molecular mass of 3451 amu, and the second has 36 amino acids with 3832 amu. Both peptides are tightly bound by three disulfide bridges. TdK2 was shown to block reversibly the Shaker B K(+)-channel expressed heterologously in Sf9 cells. The systematic number assigned to TdK2 is alpha-KTx-18.2 and that of TdK3 is alpha-KTx-18.3. Comparative analysis of the amino acid sequences found suggests that this venom contains peptides highly similar to those that block K(+) channels, as well as those that modify the gating mechanisms of Na(+) channels, found in other scorpions. Additionally, peptides similar to defensins were also identified.
Authors:
Cesar V F Batista; Gina D'Suze; Froylan Gómez-Lagunas; Fernando Z Zamudio; Sergio Encarnación; Carlos Sevcik; Lourival D Possani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  6     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-13     Completed Date:  2006-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3718-27     Citation Subset:  IM    
Affiliation:
Department of Molecular Medicine and Bioprocesses, Biotechnology Institute, UNAM, Cuernavaca, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Culture Techniques
Cells, Cultured
Chromatography, High Pressure Liquid
Electrophysiology
Hydrolysis
Molecular Weight
Peptide Fragments / chemistry,  genetics,  isolation & purification
Peptide Mapping
Proteome / analysis*
Scorpion Venoms / chemistry*,  isolation & purification,  toxicity*
Scorpions / chemistry*,  genetics
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spodoptera / cytology
Toxins, Biological / chemistry*
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Peptide Fragments; 0/Proteome; 0/Scorpion Venoms; 0/Toxins, Biological; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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