Document Detail


Proteomic analysis of Herbaspirillum seropedicae cultivated in the presence of sugarcane extract.
MedLine Citation:
PMID:  23331092     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Endophytic nitrogen-fixing species of the genus Herbaspirillum colonize sugar cane and can promote plant growth. The molecular mechanisms that mediate plant- H. seropedicae interaction are poorly understood. In this work we used 2D-PAGE electrophoresis to identify H. seropedicae proteins differentially expressed at the log growth phase in the presence of sugar cane extract. The differentially expressed proteins were validated by RT qPCR. A total of 16 differential spots (1 exclusively expressed, 7 absents, 5 up- and 3 down-regulated) in the presence of 5% sugar cane extract were identified, thus the host extract is able to induce and repress specific genes of H. seropedicae. The differentially expressed proteins suggest that exposure to sugar cane extract induced metabolic changes and adaptations in H. seropedicae presumably in preparation to stablish interaction with the plant.
Authors:
Fabio Aparecido Cordeiro; Michele Zibetti Tadra-Sfeir; Luciano Fernandes Huergo; Fábio de Oliveira Pedrosa; Rose Adele Monteiro; Emanuel Maltempi de Souza
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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