| Proteomic profiling of liver from Atlantic salmon (Salmo salar) fed genetically modified soy compared to the near-isogenic non-GM line. | |
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MedLine Citation:
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PMID: 19618241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate potential differences in liver protein expression of Atlantic salmon fed genetically modified (GM) Roundup Ready soy at a high inclusion level (25% inclusion, constituting 21% of crude protein in the diet) for 7 months or a compositionally similar non-GM diet. The liver was selected as the target organ due to its importance in the general metabolism, and 2D gel electrophoresis used as a screening tool. Samples from 12 individual fish from each diet group were evaluated. Of a total of 781 analysed protein spots, only 36 were significantly different by ANOVA (p < 0.05) in abundance between the diet groups. All these spots had low fold differences (1.2-1.6) and high false discovery rate (q = 0.44), indicating minor differences in liver protein synthesis between fish fed GM and non-GM soy. Additionally, low fold differences were observed. Four protein spots were analyzed by liquid chromatography tandem mass spectrometry and identified using a combination of online searches in NCBI and searches in an inhouse database containing salmonid expressed sequence tags and contigs. Follow-up on these proteins by real-time polymerase chain reaction did not identify differences at the transcriptional level. |
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Authors:
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Nini H Sissener; Samuel A M Martin; Phillip Cash; Ernst M Hevrøy; Monica Sanden; Gro-Ingunn Hemre |
Publication Detail:
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Type: Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-17 |
Journal Detail:
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Title: Marine biotechnology (New York, N.Y.) Volume: 12 ISSN: 1436-2236 ISO Abbreviation: Mar. Biotechnol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892712 Medline TA: Mar Biotechnol (NY) Country: United States |
Other Details:
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Languages: eng Pagination: 273-81 Citation Subset: IM |
Affiliation:
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National Institute of Seafood and Nutrition Research (NIFES), Postboks 2029, Nordnes, 5817 Bergen, Norway. nsi@nifes.no |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis* Animal Nutritional Physiological Phenomena Animals Diet / veterinary* Energy Intake Food, Genetically Modified Gene Expression Regulation / drug effects Plants, Genetically Modified Proteomics* Salmo salar / metabolism* Soybeans / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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//Biotechnology and Biological Sciences Research Council |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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