Document Detail


Proteomic patterns of colonic mucosal tissues delineate Crohn's colitis and ulcerative colitis.
MedLine Citation:
PMID:  23382084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Although Crohn's colitis (CC) and ulcerative colitis (UC) share several clinical features, they have different causes, mechanisms of tissue damage, and treatment options. Therefore, the accurate diagnosis is of paramount importance in terms of medical care. The distinction between CC/UC is made on the basis of clinical, radiologic, endoscopic, and pathologic interpretations but cannot be differentiated in up to 15% of inflammatory bowel disease patients. Correct management of this "indeterminate colitis" depends on the accuracy of future, and yet not known, destination diagnosis (CC/UC).
EXPERIMENTAL DESIGN: We have developed a proteomic methodology that has the potential to discriminate between UC/CC. The histologic layers of 62 confirmed UC/CC tissues were analyzed using MALDI-MS for proteomic profiling.
RESULTS: A Support Vector Machine algorithm consisting of 25 peaks was able to differentiate spectra from CC and UC with 76.9% spectral accuracy when using a leave-20%-out cross-validation. Application of the model to the entire dataset resulted in accurate classification of 19/26 CC patients and 36/36 UC patients when using a 2/3 correct cutoff. A total of 114 peaks were found to have Wilcoxin rank sum p-values of less than 0.05.
CONCLUSION AND CLINICAL RELEVANCE: This information may provide new avenues for the development of novel personalized therapeutic targets.
Authors:
Erin H Seeley; Mary K Washington; Richard M Caprioli; Amosy E M'Koma
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-05-08
Journal Detail:
Title:  Proteomics. Clinical applications     Volume:  7     ISSN:  1862-8354     ISO Abbreviation:  Proteomics Clin Appl     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-08-07     Completed Date:  2014-06-11     Revised Date:  2014-06-25    
Medline Journal Info:
Nlm Unique ID:  101298608     Medline TA:  Proteomics Clin Appl     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  541-9     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Biological Markers / metabolism
Colitis, Ulcerative / metabolism,  pathology*,  surgery
Colon / metabolism*,  pathology*
Crohn Disease / metabolism,  pathology*,  surgery
Endoscopy, Gastrointestinal
Female
Humans
Intestinal Mucosa / metabolism*,  pathology*
Male
Middle Aged
Proteomics*
Young Adult
Grant Support
ID/Acronym/Agency:
1 UL1 RR024975/RR/NCRR NIH HHS; 3U54 CA091405-09S1/CA/NCI NIH HHS; 5P 30 DK58404-08/DK/NIDDK NIH HHS; 5R01-GM58008/GM/NIGMS NIH HHS; 5U 01CA094664-09/CA/NCI NIH HHS; 5U54RR026140-03/RR/NCRR NIH HHS; NIH/NCI-CA068485/CA/NCI NIH HHS; P50CA095103/CA/NCI NIH HHS; R21 DK095186/DK/NIDDK NIH HHS; U54 CA091405/CA/NCI NIH HHS; U54 CA163066/CA/NCI NIH HHS; U54 CA163069/CA/NCI NIH HHS; U54 CA163072/CA/NCI NIH HHS; U54 MD007593/MD/NIMHD NIH HHS; U54 RR026140/RR/NCRR NIH HHS; UL1 RR024975/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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