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Proteomic mechanism of myocardial angiogenesis augmented by remote ischemic training of skeletal muscle in rabbit.
MedLine Citation:
PMID:  20406236     Owner:  NLM     Status:  In-Data-Review    
Aims: This study was designed to evaluate the proteomic mechanism of myocardial angiogenesis augmented by a remote ischemic training (RIT) of skeletal muscle in a controlled myocardial ischemia model. Methods: The rabbits were grouped by RIT, myocardial ischemia without RIT (MI), and sham-operation (Sham). Controlled myocardial ischemia was modeled by a balloon constrictor implanted on their left ventricular branch (LVB) in a New Zealand rabbit. RIT was induced by four cycles of 10-minute ischemia followed by 10 minutes of reperfusion using tourniquets on the hind limbs of the myocardial ischemia models for 4 weeks. The myocardial samples were subjected to two dimensional electrophoresis and MALDI TOF for protein identification. The angiogenesis was documented by using microspheres to measure the relative regional blood flow, immunohistochemistry to assess capillary density (Factor VIII), and Western blotting to measure the vascular endothelial growth factor (VEGF) levels. Results: Thirty-eight differentially expressed protein spots between RIT and MI were separated by two-dimensional gel electrophoresis, and of those, 22 proteins were identified by mass spectrometry. The regional blood flow, capillary density and VEGF in RIT were 35%, 49% and 28% higher than MI (p < 0.01). The increase of regional blood flow and capillary density were highly correlated with VEGF (r= 0.74 and r= 0.67, respectively; p < 0.01). Both RIT and MI groups exhibited stronger angiogenesis than sham treatment (p < 0.01). Conclusions: Augmentation of angiogenesis in ischemic myocardium by RIT has identical identified proteomic findings with differentially expressed proteins.
Jing Gao; Mei Shen; Xuejiang Guo; Xiaojie Li; Jianan Li
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Publication Detail:
Type:  Journal Article     Date:  2010-04-07
Journal Detail:
Title:  Cardiovascular therapeutics     Volume:  29     ISSN:  1755-5922     ISO Abbreviation:  Cardiovasc Ther     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101319630     Medline TA:  Cardiovasc Ther     Country:  England    
Other Details:
Languages:  eng     Pagination:  199-210     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China Department of Child Rehabilitation, Huaian Maternal and Child Health Care Hospital, Huaian, China Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China Department of Rehabilitation Medicine, the Third Affiliated Hospital of Jiamusi Medical University, Jiamusi, China.
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