Document Detail

Proteomic Identification Network Analysis of Haptoglobin as a Key Regulator Associated with Liver Fibrosis.
MedLine Citation:
PMID:  23274719     Owner:  NLM     Status:  Publisher    
Liver fibrosis (LF) is the final stage of liver dysfunction, characterized by diffuse fibrosis which is the main response to the liver injury. Haptoglobin (HP) protein, produced as an acute phase reactant during LF, preventing liver damage, may be potential molecular targets for early LF diagnostics and therapeutic applications. However, protein networks associated with the HP are largely unknown. To address this issue, we used a pathological mouse model of LF that was induced by treatment with carbon tetrachloride for 8 days. HP protein was separated and identified by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. HP protein was subjected to functional pathway analysis using STRING and Cytoscape software for better understanding of the protein-protein interaction (PPI) networks in biological context. Bioinformatics analyses revealed that HP expression associated with fibrosis was upregulated, and suggested that HP responsible for fibrosis may precede the onset and progression of LF. Using the web-based database, functional pathway analysis suggested the modulation of multiple vital physiological pathways, including antioxidation immunity, signal transduction, metabolic process, energy production, cell apoptosis, oxidation reduction, DNA repair process, cell communication, and regulation of cellular process. The generation of protein interaction networks clearly enhances the interpretation and understanding of the molecular mechanisms of HP. HP protein represents targets for further experimental investigation that will provide biological insight and potentially could be exploited for novel therapeutic approaches to combat LF.
Aihua Zhang; Hui Sun; Wejun Sun; Yuan Ye; Xijun Wang
Related Documents :
6158299 - Serum protein changes in experimentally induced amyloidosis in hamsters.
2324879 - Neonatal serum protein levels as indicators of nutritional status: normal values and co...
24301779 - Isolation and identification of bovine primary hepatocytes.
11885509 - Protein profile in leprosy.
23426619 - Detection of tyrosine‑phosphorylated proteins in hepatocellular carcinoma tissues usi...
21855799 - Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase chip.
18178719 - Microtubule-dependent distribution of mrna in adult cardiocytes.
17465519 - A fragment-based approach for the discovery of isoform-specific p38alpha inhibitors.
15379559 - Kinetic and structural characterization of slr0077/sufs, the essential cysteine desulfu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
National TCM Key Lab of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, and Key Pharmacometabolomics Platform of Chinese Medicines, Heping Road 24, Harbin, 150040, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A modified ingrowth core method for measuring fine root production, mortality and decomposition in f...
Next Document:  In Silico and Experimental Characterization of Chimeric Bacillus thermocatenulatus Lipase with the C...